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PMID: 6268802 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of AKR murine leukemia virus sequences in AKR mouse substrains and structure of integrated recombinant genomes in tumor tissues.

Journal of virology ·Vol. 39 ·No. 1 ·1981-07-00 ·Pages 1-10

Quint W, Quax W, van der Putten H, Berns A

Abstract

A specific cDNA probe of AKR murine leukemia virus (AKR-MLV) was prepared to detect AKR-MLV sequences in normal and tumor tissues in a variety of AKR mouse substrains. AKR strains contained up to six endogenous AKR-MLV genomes. All substrains tested had one AKR-MLV locus in common, and closely related substrains had several proviruses integrated in an identical site. Virus-induced tumors in the AKR/FuRdA and AKR/JS strains showed a reintegration pattern of AKR-MLV sequences unique for the individual animal, suggesting a monoclonal origin for the outgrown tumors. An analysis of tumor DNAs from the AKR/FuRdA and AKR/JS substrains with restriction enzymes cleaving within the proviral genome revealed a new EcoRI restriction site and BamHI restriction site not present in normal tissues. The positions of these sites corresponded both with cleavage sites of EcoRI and BamHI in integrated Moloney recombinants and with the structure of isolated AKR mink cell focus-forming viruses. All tumors analyzed to data contain nearly identical integrated recombinant genomes, suggesting a causal relationship between the formation of recombinants and the leukemogenic process.

MeSH Terms
AKR murine leukemia virus/genetics Animals Base Sequence DNA DNA Restriction Enzymes DNA, Neoplasm/analysis Genes, Viral Leukemia Virus, Murine/genetics Leukemia, Experimental/analysis Mice Mice, Inbred AKR Nucleic Acid Hybridization Recombination, Genetic
Chemicals
DNA, Neoplasm DNA DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Quint W
Quax W
van der Putten H
Berns A
References (32)
32 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.39.1.1-10.1981
Published
1981-07-00
Pages
1-10
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC171259
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.39.1.1-10.1981 PMC Full Text View journal details
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