Home LiteratureArticle Details
PMID: 4372624 Published · ppublish English Journal Article

AKR murine leukemia virus genome: frequency of sequences in DNA of high-, low-, and non-virus-yielding mouse strains.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 9 ·1974-09-00 ·Pages 3555-9

Lowy DR, Chattopadhyay SK, Teich NM, Rowe WP, Levine AS

Abstract

Studies with a single-stranded DNA probe complementary to the RNA of mouse-tropic AKR murine leukemia virus indicate that the complete genome of the AKR-type murine leukemia virus is present in the DNA of high- and low-virus-yielding mouse strains, while DNA of non-virus-yielding strains contains only a part of the genome. Furthermore, in those strains where the genome is complete, two populations of virus-specific DNA sequences can be identified (more abundant and less abundant species) according to their rate of association with the probe. Low-virus-yielding mouse strains contain fewer copies of the less abundant species and, consequently, fewer complete viral genomes than do high-virus-yielding strains. Thus, in the ten strains tested, there is a good correlation between completeness of the genome of AKR-type murine leukemia virus in cellular DNA and the capacity of the cells to release infectious AKR-type murine leukemia virus. Moreover, the number of complete viral genomes correlates with the frequency of infectious virus production by virus-positive strains. DNA from wild Mus musculus also contained viral sequences, the sample tested showing reassociation kinetics identical to the non-virus-producing strains.

MeSH Terms
Animals Base Sequence DNA, Single-Stranded DNA, Viral/metabolism Genotype Kinetics Leukemia Virus, Murine/metabolism Mice Mice, Inbred AKR Nucleic Acid Hybridization RNA, Viral Temperature Tritium Virus Replication
Chemicals
DNA, Single-Stranded DNA, Viral RNA, Viral Tritium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lowy D R
Chattopadhyay S K
Teich N M
Rowe W P
Levine A S
References (27)
27 references, click to expand
  1. Kinetics of renaturation of DNA.
    J Mol Biol. 1968 Feb 14;31(3):349-70 PMID: 5637197
  2. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms.
    Science. 1968 Aug 9;161(3841):529-40 PMID: 4874239
  3. Isolation and characterization of bacterial ribosomal RNA cistrons.
    Biophys J. 1968 Oct;8(10):1104-18 PMID: 4878345
  4. Evolution of higher-organism DNA.
    Q Rev Biophys. 1970 Aug;3(3):327-75 PMID: 4989149
  5. Demonstration of biological activity of a murine leukemia virus of New Zealand black mice.
    Science. 1970 Oct 16;170(3955):326-7 PMID: 4318971
  6. Heterogeneity of murine leukemia virus in vitro DNA; detection of viral DNA in mammalian cells.
    Science. 1971 Jun 25;172(3990):1353-5 PMID: 4325521
  7. Murine leukemia virus: high-frequency activation in vitro by 5-iododeoxyuridine and 5-bromodeoxyuridine.
    Science. 1971 Oct 8;174(4005):155-6 PMID: 4330367
  8. Induction of murine C-type viruses from clonal lines of virus-free BALB-3T3 cells.
    Science. 1971 Oct 8;174(4005):157-9 PMID: 5119627
  9. A crude nuclease preparation suitable for use in DNA reassociation experiments.
    Biochim Biophys Acta. 1971 Jul 29;240(4):522-31 PMID: 5123563
  10. Noninfectious AKR mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious murine leukemia virus.
    Virology. 1971 Dec;46(3):866-76 PMID: 4332980
  11. Detection of avian tumor virus-specific nucleotide sequences in avian cell DNAs (reassociation kinetics-RNA tumor viruses-gas antigen-Rous sarcoma virus, chick cells).
    Proc Natl Acad Sci U S A. 1972 Jan;69(1):20-4 PMID: 4333039
  12. Quantitative studies of naturally occurring murine leukemia virus infection of AKR mice.
    J Exp Med. 1972 Feb 1;135(2):429-36 PMID: 4334098
  13. Widespread presence, in chickens, of DNA complementary to the RNA genome of avian leukosis viruses.
    Proc Natl Acad Sci U S A. 1972 Mar;69(3):576-80 PMID: 4335067
  14. Studies of genetic transmission of murine leukemia virus by AKR mice. I. Crosses with Fv-1 n strains of mice.
    J Exp Med. 1972 Nov 1;136(5):1272-85 PMID: 4343244
  15. Studies of genetic transmission of murine leukemia virus by AKR mice. II. Crosses with Fv-1 b strains of mice.
    J Exp Med. 1972 Nov 1;136(5):1286-301 PMID: 4343245
  16. RNA in mammalian sarcoma virus transformed nonproducer cells homologous to murine leukemia virus RNA.
    Virology. 1973 Feb;51(2):370-82 PMID: 4120907
  17. Segregation of loci for C-type virus induction in strains of mice with high and low incidence of leukemia.
    Science. 1973 May 25;180(4088):865-6 PMID: 4122421
  18. Measurement of endogenous leukosis virus nucleotide sequences in the DNA of normal avian embryos by RNA-DNA hybridization.
    Virology. 1973 May;53(1):196-203 PMID: 4350417
  19. A type-C virus in human rhabdomyosarcoma cells after inoculation into NIH Swiss mice treated with antithymocyte serum.
    Proc Natl Acad Sci U S A. 1973 Mar;70(3):859-62 PMID: 4123693
  20. Characterization of murine leukaemia virus-specific DNA present in normal mouse cells.
    Nat New Biol. 1973 Jul 18;244(133):76-9 PMID: 4352888
  21. Independent segregation of loci for activation of biologically distinguishable RNA C-type viruses in mouse cells.
    Proc Natl Acad Sci U S A. 1973 Jul;70(7):2055-8 PMID: 4352968
  22. Appearance of virus-specific DNA in mammalian cells following transformation by Rous sarcoma virus.
    J Mol Biol. 1973 Mar 15;74(4):613-26 PMID: 4125755
  23. Xenotropic viruses: murine leukemia viruses associated with NIH Swiss, NZB, and other mouse strains.
    Science. 1973 Dec 14;182(4117):1151-3 PMID: 4356281
  24. Genetic factors in the natural history of murine leukemia virus infection: G. H. A. Clowes Memorial Lecture.
    Cancer Res. 1973 Dec;33(12):3061-8 PMID: 4357347
  25. Primate and murine type-C viral nucleic acid association kinetics: analysis of model systems and natural tissues.
    J Virol. 1974 Feb;13(2):363-9 PMID: 4359298
  26. Evidence that the AKR murine-leukemia-virus genome is complete in DNA of the high-virus AKR mouse and incomplete in the DNA of the "virus-negative" NIH mouse.
    Proc Natl Acad Sci U S A. 1974 Jan;71(1):167-71 PMID: 4359326
  27. RNA-directed DNA synthesis by virions of Rous sarcoma virus: further characterization of the templates and the extent of their transcription.
    Virology. 1973 Mar;52(1):264-74 PMID: 4139806
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.9.3555
Published
1974-09-00
Pages
3555-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433813
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.9.3555 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2