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PMID: 3025832 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning and characterization of the high-affinity cAMP phosphodiesterase of Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 24 ·1986-12-00 ·Pages 9303-7

Sass P, Field J, Nikawa J, Toda T, Wigler M

Abstract

A gene, PDE2, has been cloned from the yeast Saccharomyces cerevisiae that, when present in high copy, reverses the phenotypic effects of RAS2Val19, a mutant form of the RAS2 gene that renders yeast cells sensitive to heat shock and starvation. It has previously been shown that the RAS proteins are potent activators of yeast adenylate cyclase. We report here that PDE2 encodes a high-affinity cAMP phosphodiesterase that shares sequence homology with animal cell phosphodiesterases. These results therefore imply that the effects of RAS2Val19 are mediated through its changes in cAMP concentration.

MeSH Terms
3',5'-Cyclic-AMP Phosphodiesterases/genetics Amino Acid Sequence Base Sequence Cell Cycle Cloning, Molecular Cyclic AMP/metabolism DNA, Fungal/genetics Gene Expression Regulation Genes, Fungal Hot Temperature Phenotype Saccharomyces cerevisiae/cytology,enzymology,genetics
Chemicals
DNA, Fungal Cyclic AMP 3',5'-Cyclic-AMP Phosphodiesterases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sass P
Field J
Nikawa J
Toda T
Wigler M
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33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.24.9303
Published
1986-12-00
Pages
9303-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC387126
Subset
IM
Databases
GENBANK
M14563
External Links
PubMed source DOI: 10.1073/pnas.83.24.9303 PMC Full Text View journal details
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