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PMID: 3025834 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular analysis of cDNA clones and the corresponding genomic coding sequences of the Drosophila dunce+ gene, the structural gene for cAMP phosphodiesterase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 24 ·1986-12-00 ·Pages 9313-7

Chen CN, Denome S, Davis RL

Abstract

We have isolated and sequenced cDNA clones representing portions of the polyadenylylated transcripts of the dunce+ gene. These define an open reading frame of 1086 bases and some of the 5'- and 3'-untranslated regions of the transcripts. The deduced amino acid sequence is strikingly homologous to the amino acid sequence of a Ca2+/calmodulin-dependent cyclic nucleotide phosphodiesterase isolated from bovine brain and more weakly related to the predicted amino acid sequence of a yeast cAMP phosphodiesterase. These homologies, together with prior genetic and biochemical studies, provide unambiguous evidence that dunce+ codes for a phosphodiesterase. In addition, the dunce+ gene product shares a seven-amino acid sequence with a regulatory subunit of cAMP-dependent protein kinase that is predicted to be part of the cAMP binding site. We also identify a weak homology between a region of the dunce+ gene product and the egg-laying hormone precursor of Aplysia californica. The open reading frame is divided in the genome by four introns.

MeSH Terms
3',5'-Cyclic-AMP Phosphodiesterases/genetics Aplysia/genetics Base Sequence Binding Sites Cloning, Molecular Cyclic AMP/metabolism DNA/genetics Drosophila melanogaster/enzymology,genetics Genes Invertebrate Hormones/genetics Learning/physiology Protein Kinases/genetics Sequence Homology, Nucleic Acid
Chemicals
Invertebrate Hormones egg-laying hormone, Mollusca DNA Cyclic AMP Protein Kinases 3',5'-Cyclic-AMP Phosphodiesterases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chen C N
Denome S
Davis R L
References (34)
34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.24.9313
Published
1986-12-00
Pages
9313-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC387128
Subset
IM
Grants
NINDS NIH HHS · NS-19904 · United States
Databases
GENBANK
M14977, M14978, M14979, M14980, M14981, M14982
External Links
PubMed source DOI: 10.1073/pnas.83.24.9313 PMC Full Text View journal details
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