Adenovirus 2 peptide IX gene is expressed only on replicated DNA molecules.

Molecular and cellular biology ·Vol. 6 ·No. 12 ·1986-12-00 ·Pages 4149-54

Matsui T, Murayama M, Mita T

Abstract

Transcription of the adenovirus type 2 peptide IX (pIX) gene was examined in transient expression assays. When a nonreplicating plasmid DNA containing the pIX gene was introduced into HeLa cells by the DEAE-dextran method, no pIX gene transcript was detected. In contrast, efficient transcription was observed in the cells transfected with a replicating plasmid containing the pIX gene. Adenovirus early genes did not affect the level of transcription of the pIX gene on either a nonreplicating or a replicating plasmid. Inhibition of plasmid replication with cytosine arabinoside prevented transcription of the pIX gene. By quantitative analysis of the amount of the pIX gene and its transcript in transfected cells, it was concluded that active transcription of the pIX gene occurred only on DNA molecules replicated in the cell.

MeSH Terms

Adenoviruses, Human/genetics DNA Replication DNA Restriction Enzymes DNA, Viral/genetics Genes Genes, Viral HeLa Cells/metabolism Humans Kinetics Nucleic Acid Hybridization Plasmids Transfection Viral Proteins/genetics

Chemicals

DNA, Viral Viral Proteins DNA Restriction Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Matsui T

Murayama M

Mita T

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31 references, click to expand

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Article Info

Journal

Molecular and cellular biology

Abbr.

Mol Cell Biol

ISSN

0270-7306

DOI

10.1128/mcb.6.12.4149-4154.1986

Published

1986-12-00

Pages

4149-54

Language

English

Region

United States

NLM ID

8109087

PMCID

PMC367193

Subset

External Links

PubMed source DOI: 10.1128/mcb.6.12.4149-4154.1986 PMC Full Text View journal details

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Adenovirus 2 peptide IX gene is expressed only on replicated DNA molecules.

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