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PMID: 3023301 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

T-DNA and opine synthetic loci in tumors incited by Agrobacterium tumefaciens A281 on soybean and alfalfa plants.

Journal of bacteriology ·Vol. 168 ·No. 3 ·1986-12-00 ·Pages 1283-90

Hood EE, Chilton WS, Chilton MD, Fraley RT

Abstract

We report here the molecular characterization of transferred DNA (T-DNA) in leguminous tumors incited by Agrobacterium tumefaciens A281 harboring the tumor-inducing plasmid pTiBo542. The T-DNA is composed of two regions named TL (left portion)-DNA and TR (right portion)-DNA, in accordance with the nomenclature for the octopine strains. TL-DNA is defined by several internal HindIII restriction fragments totaling 10.8 kilobase pairs (kbp) in uncloned soybean and alfalfa tumors. Alfalfa tumor DNA may contain one more HindIII fragment at the left end of TL-DNA than does soybean tumor DNA. TR-DNA has a 5.8-kbp BamHI-EcoRI internal fragment. All borders other than the left border of TL-DNA appear to be the same within the detection limits of Southern blot hybridization experiments. The two T-DNA regions are separated by 16 to 19 kbp of DNA not stably maintained in tumors. The distance from the left border of TL-DNA to the right border of TR-DNA is approximately 40 kbp. Loci for the mannityl opines are situated in TR-DNA, based on genetic and biochemical criteria.

MeSH Terms
Cloning, Molecular DNA Restriction Enzymes DNA, Bacterial/analysis DNA, Neoplasm/analysis Genes, Bacterial Mannitol/analogs & derivatives,genetics Medicago sativa/analysis Plant Tumors/analysis Plasmids Rhizobium/genetics Soybeans/analysis
Chemicals
DNA, Bacterial DNA, Neoplasm Mannitol DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hood E E
Chilton W S
Chilton M D
Fraley R T
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38 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.168.3.1283-1290.1986
Published
1986-12-00
Pages
1283-90
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213635
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.168.3.1283-1290.1986 PMC Full Text View journal details
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