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PMID: 3007112 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In vitro transcription by Xenopus oocytes RNA polymerase III requires a DNA topoisomerase II activity.

The EMBO journal ·Vol. 5 ·No. 1 ·1986-01-00 ·Pages 151-5

Glikin GC, Blangy D

Abstract

Extracts of Xenopus laevis oocytes are able to assemble minichromosomes in vitro when they are supplemented with ATP and Mg2+. We have followed the time course of in vitro DNA supercoiling and transcription of polyoma virus closed circular DNA. The transcriptional activity increased with the assembly time of chromatin in the presence of both ATP and Mg2+, but only residual activity was detected in the absence of either of them. We also found that polyoma DNA as well as 5S RNA gene transcription were carried out mainly by an RNA polymerase III. On the other hand, polyoma RNA and 5S RNA synthesis were inhibited by novobiocin in a way that suggested the requirement of a DNA topoisomerase II or DNA gyrase activity for the initiation of transcription.

MeSH Terms
Animals Chromatin/metabolism DNA Topoisomerases, Type II/metabolism DNA, Superhelical/metabolism DNA, Viral/genetics DNA-Directed RNA Polymerases/metabolism Embryo, Mammalian Embryo, Nonmammalian Female Mice Novobiocin/pharmacology Oocytes/metabolism Polyomavirus/genetics RNA Polymerase III/metabolism Transcription, Genetic/drug effects Xenopus
Chemicals
Chromatin DNA, Superhelical DNA, Viral Novobiocin DNA-Directed RNA Polymerases RNA Polymerase III DNA Topoisomerases, Type II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Glikin G C
Blangy D
References (37)
37 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-01-00
Pages
151-5
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1166707
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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