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PMID: 2987817 Published · ppublish English Journal Article

DNAaseI-hypersensitive minichromosomes of SV40 possess an elastic torsional strain in DNA.

Nucleic acids research ·Vol. 13 ·No. 4 ·1985-02-25 ·Pages 1135-49

Luchnik AN, Bakayev VV, Yugai AA, Zbarsky IB, Georgiev GP

Abstract

Previously, we have shown that DNA in a small fraction (2-5%) of SV40 minichromosomes was torsionally strained and could be relaxed by treating minichromosomes with topoisomerase I. This fraction was enriched with endogeneous RNA polymerase II (Luchnik et al., 1982, EMBO J., 1, 1353). Here we show that one and the same fraction of SV40 minichromosomes is hypersensitive to DNAase I and is relaxable by topoisomerase I. Moreover, this fraction completely loses its hypersensitivity to DNAase I upon relaxation. The possibility that this fraction of minichromosomes can be represented by naked DNA is ruled out by the results of studying the kinetics of minichromosome digestion by DNAase I in comparison to digestion of pure SV40 DNA and by measuring the buoyant density of SV40 chromatin in equilibrium CsCl gradient. Our data obtained with SV40 minichromosomes may be relevant to the mechanism responsible for DNAase I hypersensitivity in the loops or domains of cellular chromatin.

MeSH Terms
Animals Cells, Cultured Chlorocebus aethiops Chromatin/physiology,ultrastructure DNA, Superhelical/genetics DNA, Viral/genetics Deoxyribonuclease I Gene Expression Regulation Nucleic Acid Conformation Simian virus 40/genetics
Chemicals
Chromatin DNA, Superhelical DNA, Viral Deoxyribonuclease I
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Luchnik A N
Bakayev V V
Yugai A A
Zbarsky I B
Georgiev G P
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.4.1135
Published
1985-02-25
Pages
1135-49
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341061
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/13.4.1135 PMC Full Text View journal details
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