Home LiteratureArticle Details
PMID: 216012 Published · ppublish English Journal Article

DNAs of simian virus 40 and polyoma direct the synthesis of viral tumor antigens and capsid proteins in Xenopus oocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 12 ·1978-12-00 ·Pages 6073-7

Rungger D, Türler H

Abstract

Purified simian virus 40 and polyoma DNAs injected into nuclei of Xenopus oocytes were transcribed and subsequently translated into virus-specific tumor antigens and capsid proteins. Simian virus 40 large and small tumor antigens synthesized in the oocytes were indistinguishable, by gel electrophoresis and [35S]methionine-labeled tryptic peptide mapping, from the corresponding polypeptides synthesized in CV-1 African green monkey cells. The synthesis of large simian virus 40 tumor antigen implies the correct splicing of its mRNA, which is complementary to nonadjacent nucleotide sequences in the early region of the viral genome. Polyoma DNA directed synthesis of two polyoma tumor antigen polypeptides, 57,000 Mr and small tumor antigen, and of the main capsid protein.

MeSH Terms
Animals Antigens, Neoplasm/genetics Antigens, Viral/genetics DNA, Viral/genetics Female Molecular Weight Oocytes/metabolism Polyomavirus/genetics,immunology Protein Biosynthesis Simian virus 40/genetics,immunology Transcription, Genetic Viral Proteins/genetics Xenopus
Chemicals
Antigens, Neoplasm Antigens, Viral DNA, Viral Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rungger D
Türler H
References (36)
36 references, click to expand
  1. Tumor antigen(s) in cell productively infected by wild-type polyoma virus and mutant NG-18.
    Proc Natl Acad Sci U S A. 1978 Jan;75(1):79-83 PMID: 203944
  2. Changes in somatic cell nuclei inserted into growing and maturing amphibian oocytes.
    J Embryol Exp Morphol. 1968 Nov;20(3):401-14 PMID: 5750105
  3. Complete nucleotide sequence of SV40 DNA.
    Nature. 1978 May 11;273(5658):113-20 PMID: 205802
  4. The genome of simian virus 40.
    Science. 1978 May 5;200(4341):494-502 PMID: 205947
  5. Coupled transcription-translation of DNA injected into Xenopus oocytes.
    Cell. 1977 Sep;12(1):175-82 PMID: 198138
  6. Purified DNAs are transcribed after microinjection into Xenopus oocytes.
    Proc Natl Acad Sci U S A. 1977 Apr;74(4):1502-6 PMID: 193103
  7. Characterization of polyoma virus T antigen.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):1259-63 PMID: 191834
  8. Cell-free translation of simian virus 40 early messenger RNA coding for viral T-antigen.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):457-61 PMID: 191809
  9. Identification of simian virus 40 protein A.
    J Virol. 1977 Feb;21(2):636-46 PMID: 189091
  10. Synthesis of the tumour antigen and the major capsid protein of simian virus 40 in a cell-free system derived from Escherichia coli.
    J Mol Biol. 1976 Dec;108(2):361-79 PMID: 189036
  11. The effect of heat shock on gene expression in Drosophila melanogaster.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 2:819-27 PMID: 98271
  12. Transcription of xenopus tDNAmet1 and sea urchin histone DNA injected into the Xenopus oocyte nucleus.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 2:1077-82 PMID: 277302
  13. Spliced segments at the 5' terminus of adenovirus 2 late mRNA.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3171-5 PMID: 269380
  14. Transcription of cloned tRNA gene fragments and subfragments injected into the oocyte nucleus of Xenopus laevis.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1176-80 PMID: 274710
  15. Gene activation in somatic nuclei after injection into amphibian oocytes.
    Proc Natl Acad Sci U S A. 1977 Jun;74(6):2470-4 PMID: 267940
  16. High-fidelity transcription of 5S DNA injected into Xenopus oocytes.
    Proc Natl Acad Sci U S A. 1977 May;74(5):2064-8 PMID: 266727
  17. Translational control of globin synthesis by haemin in Xenopus oocytes.
    Nat New Biol. 1973 Nov 28;246(152):99-102 PMID: 4518941
  18. Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
    J Morphol. 1972 Feb;136(2):153-79 PMID: 4109871
  19. Characterization of polypeptides made in vitro from bacteriophage lambda DNA.
    J Mol Biol. 1973 Aug 25;78(4):589-600 PMID: 4762922
  20. Characterization of the single-strand-specific nuclease S1 activity on double-stranded supercoiled polyoma DNA.
    Eur J Biochem. 1974 Apr 16;43(3):591-600 PMID: 4364862
  21. Cell-free translation of messenger RNA of simian virus 40: synthesis of the major capsid protein.
    Proc Natl Acad Sci U S A. 1974 Feb;71(2):302-6 PMID: 4360938
  22. Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon.
    J Virol. 1972 Oct;10(4):591-8 PMID: 4343541
  23. Polyoma gene function required for viral DNA synthesis.
    Virology. 1973 Sep;55(1):127-35 PMID: 4353950
  24. A study on the mechanism of polyoma-induced activation of the cellular DNA-synthesizing apparatus. Synchronization by FUdR of virus-induced DNA synthesis.
    Arch Gesamte Virusforsch. 1968;24(1):1-29 PMID: 4300902
  25. The effect of arabinofuranosylcytosine on the growth cycle of simian virus 40.
    Virology. 1965 Dec;27(4):490-5 PMID: 4285374
  26. Direct biochemical mapping of eukaryotic viral DNA by means of a linked transcription-translation cell-free system.
    Proc Natl Acad Sci U S A. 1976 Aug;73(8):2747-51 PMID: 183208
  27. Two forms of simian-virus-40-specific T-antigen in abortive and lytic infection.
    Proc Natl Acad Sci U S A. 1976 Apr;73(4):1097-101 PMID: 177974
  28. A simplifying concept in tumor virology: virus-specific "pleiotropic effectors".
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:381-95 PMID: 169084
  29. Injected nuclei in frog oocytes provide a living cell system for the study of transcriptional control.
    Nature. 1976 Mar 11;260(5547):116-20 PMID: 943701
  30. Comparison of exogenous energy sources for in vitro maintenance of follicle cell-free Xenopus laevis oocytes.
    In Vitro. 1976 Mar;12(3):173-9 PMID: 944165
  31. Protein synthesis in oocytes of Xenopus laevis is not regulated by the supply of messenger RNA.
    Cell. 1977 Jun;11(2):345-51 PMID: 560911
  32. Chromatin assembly and transcription in eggs and oocytes of Xenopus laevis.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 1:171-8 PMID: 209936
  33. Translation of the messenger RNA for rabbit uteroglobin in Xenopus oocytes.
    FEBS Lett. 1975 Nov 15;59(2):305-9 PMID: 1227946
  34. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
    Eur J Biochem. 1975 Aug 15;56(2):335-41 PMID: 1175627
  35. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  36. Organization and expression of early genes of simian virus 40.
    Proc Natl Acad Sci U S A. 1978 Jan;75(1):117-21 PMID: 203926
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.12.6073
Published
1978-12-00
Pages
6073-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393120
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.12.6073 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2