Home LiteratureArticle Details
PMID: 3001719 Published · ppublish English Journal Article

The gene for human p53 cellular tumor antigen is located on chromosome 17 short arm (17p13).

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 1 ·1986-01-00 ·Pages 130-4

McBride OW, Merry D, Givol D

Abstract

A clone that cross-hybridizes with a mouse p53 probe has been isolated from a cDNA library of simian virus 40-transformed human fibroblasts. This cloned human p53 cDNA was used as a probe to examine DNAs obtained from human-rodent somatic cell hybrids that have segregated human chromosomes. The results show that the human p53 gene is located on chromosome 17. In addition, Southern analysis of hybrids prepared from human cells containing a chromosome 17 translocation allowed regional localization of the human p53 gene to the most distal band on the short arm of this chromosome (17p13). Localization of the p53 gene to 17p13 was confirmed by in situ hybridization of metaphase spreads with the human p53 probe.

MeSH Terms
Animals Antigens, Neoplasm/genetics Cell Line Cell Transformation, Viral Chromosome Mapping Chromosomes, Human, 16-18 DNA/genetics Fibroblasts Humans Hybrid Cells Mice Nucleic Acid Hybridization Simian virus 40
Chemicals
Antigens, Neoplasm DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McBride O W
Merry D
Givol D
References (40)
40 references, click to expand
  1. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.
    Mol Cell Biol. 1983 Feb;3(2):280-9 PMID: 6300662
  2. Localization of human variable and constant region immunoglobulin heavy chain genes on subtelomeric band q32 of chromosome 14.
    Nucleic Acids Res. 1982 Dec 20;10(24):8155-70 PMID: 6819544
  3. Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating oncogenes.
    Nature. 1983 Aug 18-24;304(5927):596-602 PMID: 6308472
  4. Cellular oncogenes and multistep carcinogenesis.
    Science. 1983 Nov 18;222(4625):771-8 PMID: 6356358
  5. Analysis of recombinant DNA clones specific for the murine p53 cellular tumor antigen.
    EMBO J. 1983;2(10):1633-9 PMID: 6315396
  6. A single gene and a pseudogene for the cellular tumour antigen p53.
    Nature. 1983 Dec 8-14;306(5943):594-7 PMID: 6646235
  7. Report of the Committee on the Genetic Constitution of Chromosomes 13, 14, 15, 16 and 17.
    Cytogenet Cell Genet. 1984;37(1-4):127-54 PMID: 6360556
  8. Report of the Committee on Comparative Mapping.
    Cytogenet Cell Genet. 1984;37(1-4):312-39 PMID: 6360563
  9. The 53,000-dalton cellular protein and its role in transformation.
    Int Rev Exp Pathol. 1983;25:1-50 PMID: 6321377
  10. Role of the p53 protein in cell proliferation as studied by microinjection of monoclonal antibodies.
    Mol Cell Biol. 1984 Feb;4(2):276-81 PMID: 6366518
  11. Chromosomal assignment of the murine gene encoding the transformation-related protein p53.
    Mol Cell Biol. 1984 Feb;4(2):383-5 PMID: 6366521
  12. Growth regulation of a cellular tumour antigen, p53, in nontransformed cells.
    Nature. 1984 Mar 8-14;308(5955):199-201 PMID: 6366574
  13. Chromosomal localisation of the human homologues to the oncogenes erbA and B.
    EMBO J. 1984 Jan;3(1):159-63 PMID: 6323162
  14. Ph1-positive acute leukemia with a 17q;21q translocation.
    Cancer Genet Cytogenet. 1984 May;12(1):51-5 PMID: 6713358
  15. A human c-erbA oncogene homologue is closely proximal to the chromosome 17 breakpoint in acute promyelocytic leukemia.
    Proc Natl Acad Sci U S A. 1984 Jul;81(14):4495-9 PMID: 6589608
  16. Analysis of the gene coding for the murine cellular tumour antigen p53.
    EMBO J. 1984 Sep;3(9):2179-83 PMID: 6092064
  17. The gene and the pseudogene for mouse p53 cellular tumor antigen are located on different chromosomes.
    Mol Cell Biol. 1984 Aug;4(8):1638-40 PMID: 6387444
  18. Participation of p53 cellular tumour antigen in transformation of normal embryonic cells.
    Nature. 1984 Dec 13-19;312(5995):646-9 PMID: 6095116
  19. Cooperation between gene encoding p53 tumour antigen and ras in cellular transformation.
    Nature. 1984 Dec 13-19;312(5995):649-51 PMID: 6390217
  20. Cellular immortalization by a cDNA clone encoding the transformation-associated phosphoprotein p53.
    Nature. 1984 Dec 13-19;312(5995):651-4 PMID: 6095117
  21. Constitutive fragile sites and cancer.
    Science. 1984 Dec 7;226(4679):1199-204 PMID: 6239375
  22. Isolation and characterization of a human p53 cDNA clone: expression of the human p53 gene.
    EMBO J. 1984 Dec 20;3(13):3257-62 PMID: 6396087
  23. Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells.
    EMBO J. 1985 May;4(5):1251-5 PMID: 4006916
  24. The amino acid sequence of murine p53 determined from a c-DNA clone.
    Virology. 1984 Apr 30;134(2):477-82 PMID: 6400059
  25. The use of drug-resistant markers to study the hybridization of mouse fibroblasts.
    Exp Cell Res. 1966 Jan;41(1):190-6 PMID: 4952025
  26. DELETION OF THYMIDINE KINASE ACTIVITY FROM L CELLS RESISTANT TO BROMODEOXYURIDINE.
    Exp Cell Res. 1963 Aug;31:297-312 PMID: 14056989
  27. Loss of T-antigen from somatic hybrids between mouse cells and SV40-transformed human cells.
    Proc Natl Acad Sci U S A. 1968 Apr;59(4):1132-5 PMID: 4297332
  28. A rapid banding technique for human chromosomes.
    Lancet. 1971 Oct 30;2(7731):971-2 PMID: 4107917
  29. A review of enzyme polymorphism, linkage and electrophoretic conditions for mouse and somatic cell hybrids in starch gels.
    J Histochem Cytochem. 1973 Dec;21(12):1066-81 PMID: 4587225
  30. A general method for isolation of high molecular weight DNA from eukaryotes.
    Nucleic Acids Res. 1976 Sep;3(9):2303-8 PMID: 987581
  31. Mechanisms of giemsa banding of chromosomes. I. Giemsa-11 banding with azure and eosin.
    Exp Cell Res. 1976 Oct 1;102(1):85-94 PMID: 61884
  32. SV40-53K antigen: a possible role for 53K in normal cells.
    Virology. 1981 Jul 30;112(2):785-8 PMID: 6266152
  33. Chromosomal location of human kappa and lambda immunoglobulin light chain constant region genes.
    J Exp Med. 1982 May 1;155(5):1480-90 PMID: 6802926
  34. Analysis of the nonviral antigens immunoprecipitable by SV40 T antibody from SV40-transformed human/mouse hybrid cell lines.
    Virology. 1981 Oct 15;114(1):149-60 PMID: 6269282
  35. Localization of single copy DNA sequences of G-banded human chromosomes by in situ hybridization.
    Chromosoma. 1981;83(3):431-9 PMID: 7273954
  36. The myc oncogene in man and birds.
    Nature. 1982 Sep 2;299(5878):9-10 PMID: 6955599
  37. Microinjection of monoclonal antibody to protein p53 inhibits serum-induced DNA synthesis in 3T3 cells.
    Proc Natl Acad Sci U S A. 1982 Oct;79(20):6309-12 PMID: 6292898
  38. Localization of the normal allele of T24 human bladder carcinoma oncogene to chromosome 11.
    Nature. 1982 Dec 23;300(5894):773-4 PMID: 7177197
  39. Human oncogene locations and chromosome aberrations.
    Nature. 1983 Jan 27;301(5898):290-1 PMID: 6823303
  40. The chromosomal basis of human neoplasia.
    Science. 1983 Jul 15;221(4607):227-36 PMID: 6336310
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.1.130
Published
1986-01-00
Pages
130-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC322805
Subset
IM
Databases
GENBANK
K03199
External Links
PubMed source DOI: 10.1073/pnas.83.1.130 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2