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PMID: 2999067 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Escherichia coli rep gene: identification of the promoter and N terminus of the rep protein.

Journal of bacteriology ·Vol. 164 ·No. 3 ·1985-12-00 ·Pages 1004-10

Bialkowska-Hobrzanska H, Gilchrist CA, Denhardt DT

Abstract

The functional Escherichia coli rep gene, which encodes the Mr 67,000 Rep helicase, has been localized within a 2.55-kilobase sequence. Its regulatory region has been characterized by the use of rep-lacZ fusions. The direction of transcription of the rep gene is clockwise on the E. coli chromosome, as are the nearby ilvC and rho genes. The sequence of the rep control region was determined, and putative regulatory sequences were identified; no evidence for autoregulation of expression was obtained. Transcription of the gene was not enhanced during the SOS response. The location of the promoter and the beginning of the protein were confirmed by S1 nuclease mapping of the 5' end of rep mRNA and determination of the NH2-terminal sequence of the rep protein.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence DNA Restriction Enzymes/metabolism Electrophoresis, Polyacrylamide Gel Endonucleases/metabolism Escherichia coli/genetics Gene Expression Regulation Genes, Bacterial Genes, Regulator Operon Single-Strand Specific DNA and RNA Endonucleases Structure-Activity Relationship
Chemicals
Bacterial Proteins Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bialkowska-Hobrzanska H
Gilchrist C A
Denhardt D T
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33 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.164.3.1004-1010.1985
Published
1985-12-00
Pages
1004-10
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC219290
Subset
IM
Databases
GENBANK
M11055
External Links
PubMed source DOI: 10.1128/jb.164.3.1004-1010.1985 PMC Full Text View journal details
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