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PMID: 6324092 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transcription of the uvrD gene of Escherichia coli is controlled by the lexA repressor and by attenuation.

Nucleic acids research ·Vol. 11 ·No. 24 ·1983-12-20 ·Pages 8625-40

Easton AM, Kushner SR

Abstract

The nucleotide sequence of the control region and the presumptive N-terminal portion of the uvrD gene of Escherichia coli K-12 has been determined. The 1190 base pairs of DNA examined include the likely coding sequence for the first 258 amino acids of the uvrD protein. The transcription promoter for the uvrD gene was identified upstream of the protein coding region. Synthesis of messenger RNA in vitro from this promoter was inhibited by purified lexA protein. The lexA protein was found to bind downstream from the promoter at a sequence, CTGTATATATACCCAG, which is homologous to other known lexA protein binding sites. In the absence of the lexA protein, approximately half of the messages initiated in vitro at the uvrD promoter terminate after about 60 nucleotides at a sequence which resembles a rho-independent terminator. These results indicate that the uvrD gene is induced during the SOS response, and that the expression of the gene may also be regulated by transcription attenuation.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence DNA Restriction Enzymes Escherichia coli/genetics Genes Genes, Bacterial Operon Plasmids Serine Endopeptidases Transcription, Genetic
Chemicals
Bacterial Proteins LexA protein, Bacteria DNA Restriction Enzymes Serine Endopeptidases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Easton A M
Kushner S R
References (47)
47 references, click to expand
  1. Studies on radiation-sensitive mutants of E. coli. I. Mutants defective in the repair synthesis.
    Mol Gen Genet. 1968 May 3;101(3):227-44 PMID: 4879097
  2. The mutator property of uvr502 mutation affecting UV sensitivity of Escherichia coli.
    Mol Gen Genet. 1972;118(1):51-6 PMID: 4576008
  3. Improved estimation of secondary structure in ribonucleic acids.
    Nat New Biol. 1973 Nov 14;246(150):40-1 PMID: 4519026
  4. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  5. Effect of tsl mutations on Col E1 expression in a recA strain of Escherichia coli K-12.
    J Bacteriol. 1975 Aug;123(2):775-6 PMID: 1097429
  6. Transcription termination at the trp operon attenuators of Escherichia coli and Salmonella typhimurium: RNA secondary structure and regulation of termination.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4365-9 PMID: 337297
  7. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  8. Evidence for two mechanisms for DNA unwinding catalyzed by DNA helicases.
    J Biol Chem. 1979 Nov 25;254(22):11343-50 PMID: 227866
  9. Identification of the lexA gene product of Escherichia coli K-12.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6147-51 PMID: 160562
  10. Construction and characterization of a plasmid coding for a fragment of the Escherichia coli recA protein.
    Mol Gen Genet. 1979;177(1):13-22 PMID: 395410
  11. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  12. The lexA gene product represses its own promoter.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):1932-6 PMID: 6990417
  13. Sequences of the recA gene and protein.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2611-5 PMID: 6930655
  14. DNA-damaging agents stimulate gene expression at specific loci in Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2819-23 PMID: 6771759
  15. Control of recA gene RNA in E. coli: regulatory and signal genes.
    Cell. 1980 Jul;20(3):731-7 PMID: 6998563
  16. Studies on the functions of DNA helicase I and DNA helicase II of Escherichia coli.
    J Biol Chem. 1980 Oct 25;255(20):9746-52 PMID: 6107294
  17. The nucleotide sequence of the ubiquitous repetitive DNA sequence B1 complementary to the most abundant class of mouse fold-back RNA.
    Nucleic Acids Res. 1980 Mar 25;8(6):1201-15 PMID: 7433120
  18. Evidence for the inducibility of the uvrB operon.
    Nature. 1981 Jan 15;289(5794):196-8 PMID: 7005695
  19. Expression of the E. coli uvrA gene is inducible.
    Nature. 1981 Feb 26;289(5800):808-10 PMID: 6780917
  20. The E. coli beta-lactamase attenuator mediates growth rate-dependent regulation.
    Nature. 1981 Mar 19;290(5803):221-5 PMID: 7010184
  21. An inducible DNA replication-cell division coupling mechanism in E. coli.
    Nature. 1981 Apr 30;290(5809):797-9 PMID: 7012641
  22. Rho-independent termination: dyad symmetry in DNA causes RNA polymerase to pause during transcription in vitro.
    Nucleic Acids Res. 1981 Feb 11;9(3):563-77 PMID: 7012794
  23. Localization of the Tn5 transposase promoter using the cycling reaction of RNA polymerase.
    Nucleic Acids Res. 1981 Apr 24;9(8):1873-83 PMID: 6264398
  24. Rapid and efficient cosmid cloning.
    Nucleic Acids Res. 1981 Jul 10;9(13):2989-98 PMID: 6269067
  25. Purified lexA protein is a repressor of the recA and lexA genes.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4199-203 PMID: 7027255
  26. Mechanism of action of the lexA gene product.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4204-8 PMID: 7027256
  27. Translational initiation in prokaryotes.
    Annu Rev Microbiol. 1981;35:365-403 PMID: 6170248
  28. Inducibility of a gene product required for UV and chemical mutagenesis in Escherichia coli.
    Proc Natl Acad Sci U S A. 1981 Sep;78(9):5749-53 PMID: 7029544
  29. SOS induction and autoregulation of the himA gene for site-specific recombination in Escherichia coli.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):6754-8 PMID: 6796964
  30. Molecular cloning of the uvrD gene of Escherichia coli that controls ultraviolet sensitivity and spontaneous mutation frequency.
    Mol Gen Genet. 1981;184(2):191-9 PMID: 6276691
  31. The uvrB gene of Escherichia coli has both lexA-repressed and lexA-independent promoters.
    Cell. 1982 Mar;28(3):523-30 PMID: 6280873
  32. The nucleotide sequence of the bacteriocin promoters of plasmids Clo DF13 and Co1 E1: role of lexA repressor and cAMP in the regulation of promoter activity.
    Nucleic Acids Res. 1982 Mar 25;10(6):1913-28 PMID: 6281726
  33. LexA protein inhibits transcription of the E. coli uvrA gene in vitro.
    Nature. 1982 Jul 1;298(5869):96-8 PMID: 6283374
  34. The uvrD gene of E. coli encodes a DNA-dependent ATPase.
    Nature. 1982 Jul 1;298(5869):98-100 PMID: 6123951
  35. Precise location of two promoters for the beta-lactamase gene of pBR322. S1 mapping of ribonucleic acid isolated from Escherichia coli or synthesized in vitro.
    J Biol Chem. 1982 Aug 10;257(15):9205-10 PMID: 6178738
  36. The SOS regulatory system of Escherichia coli.
    Cell. 1982 May;29(1):11-22 PMID: 7049397
  37. DNA repair in Escherichia coli: identification of the uvrD gene product.
    Proc Natl Acad Sci U S A. 1982 Sep;79(18):5616-20 PMID: 6291053
  38. Cloning of the uvrD gene of E. coli and identification of the product.
    Gene. 1982 Oct;19(3):285-95 PMID: 6218013
  39. Evidence for use of rare codons in the dnaG gene and other regulatory genes of Escherichia coli.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):687-91 PMID: 6338495
  40. Attenuation control of pyrBI operon expression in Escherichia coli K-12.
    Proc Natl Acad Sci U S A. 1983 Jan;80(2):368-72 PMID: 6300835
  41. The Salmonella typhimurium LT2 uvrD gene is regulated by the lexA gene product.
    J Bacteriol. 1983 Jun;154(3):1502-4 PMID: 6304018
  42. Regulation of the rpsU-dnaG-rpoD macromolecular synthesis operon and the initiation of DNA replication in Escherichia coli K-12.
    Mol Gen Genet. 1983;189(1):48-57 PMID: 6222240
  43. Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.
    Microbiol Rev. 1983 Mar;47(1):1-45 PMID: 6343825
  44. Compilation and analysis of Escherichia coli promoter DNA sequences.
    Nucleic Acids Res. 1983 Apr 25;11(8):2237-55 PMID: 6344016
  45. Characterisation of the promoter for the LexA regulated sulA gene of Escherichia coli.
    Mol Gen Genet. 1983;189(3):400-4 PMID: 6306396
  46. The E. coli uvrD gene product is DNA helicase II.
    Mol Gen Genet. 1983;190(2):265-70 PMID: 6135974
  47. Methyl-directed repair of DNA base-pair mismatches in vitro.
    Proc Natl Acad Sci U S A. 1983 Aug;80(15):4639-43 PMID: 6308634
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.24.8625
Published
1983-12-20
Pages
8625-40
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326612
Subset
IM
Grants
NIGMS NIH HHS · GM08457 · United States
NIGMS NIH HHS · GM27797 · United States
NIGMS NIH HHS · GM28760 · United States
Databases
GENBANK
K01148, X00225, X00738
External Links
PubMed source DOI: 10.1093/nar/11.24.8625 PMC Full Text View journal details
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