In vitro synthesis of an infectious RNA from cDNA clones of human rhinovirus type 14.

Journal of virology ·Vol. 56 ·No. 2 ·1985-11-00 ·Pages 628-32

Mizutani S, Colonno RJ

Abstract

Development of a novel infectious cDNA assay is described for human rhinovirus type 14. A full-length cDNA clone of the human rhinovirus type 14 genome RNA was assembled and transcribed in vitro by using the SP6 transcription system. Transfection of HeLa cells with the nascent RNA resulted in the production of rhinovirus indistinguishable from the parental virus by both immunological and polyacrylamide gel analysis.

MeSH Terms

Cloning, Molecular DNA/genetics Gene Expression Regulation HeLa Cells Humans Molecular Weight RNA, Viral/biosynthesis Rhinovirus/genetics Transfection Virus Replication

Chemicals

RNA, Viral DNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Mizutani S

Colonno R J

References (17)

17 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.56.2.628-632.1985

Published

1985-11-00

Pages

628-32

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC252623

Subset

External Links

PubMed source DOI: 10.1128/JVI.56.2.628-632.1985 PMC Full Text View journal details

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In vitro synthesis of an infectious RNA from cDNA clones of human rhinovirus type 14.

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