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PMID: 6324172 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Membrane-dependent uridylylation of the genome-linked protein VPg of poliovirus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 24 ·1983-12-00 ·Pages 7447-51

Takegami T, Kuhn RJ, Anderson CW, Wimmer E

Abstract

A small nucleotidyl-protein has been synthesized in vitro in a membrane fraction of poliovirus-infected HeLa cells. Analyses of the nucleotides and polypeptide have shown that the nucleotidyl-protein is VPg-pUpU: the genome-linked protein of poliovirion RNA covalently bound to the first two 5'-terminal nucleotides of poliovirus RNA. Synthesis of VPg-pUpU in vitro was sensitive to nonionic detergent. We suggest that VPg-pUpU is part of the initiation complex in poliovirus RNA replication in a membranous environment.

MeSH Terms
Genes Genes, Viral HeLa Cells/metabolism Humans Poliovirus/genetics RNA, Viral/genetics Transcription, Genetic Viral Core Proteins Viral Proteins/genetics Virion/genetics
Chemicals
RNA, Viral Viral Core Proteins Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Takegami T
Kuhn R J
Anderson C W
Wimmer E
References (32)
32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.24.7447
Published
1983-12-00
Pages
7447-51
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389968
Subset
IM
Grants
NIAID NIH HHS · AI 15122 · United States
NCI NIH HHS · CA 28146 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.24.7447 PMC Full Text View journal details
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