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PMID: 2996846 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

T-antigen is the only detectable protein on the nucleosome-free origin region of isolated simian virus 40 minichromosomes.

Chromosoma ·Vol. 92 ·No. 5 ·1985-00-00 ·Pages 391-400

Weiss E, Ghose D, Schultz P, Oudet P

Abstract

A nucleosome-free region or nucleosome gap, containing the origin of replication and the transcriptional promoter elements, is observed on 20%-25% of the SV40 minichromosomes isolated at physiological ionic strength at late time during the infectious cycle. We found that this subpopulation of gapped minichromosomes was more sensitive to digestion with a variety of single-cut restriction enzymes than the rest of the minichromosomes. This increased digestibility of gapped minichromosomes allowed us to excise the gap region by concomitant digestion with Bgl I and Msp I. T-antigen was the only detectable protein bound to this isolated chromatin fragment. In particular no histones could be detected. The presence of T-antigen on the gap region was confirmed by immunoelectron microscopy. Most of the T-antigen appeared to be located on the late side of the Bgl I restriction enzyme site.

MeSH Terms
Animals Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/genetics Cell Line Chlorocebus aethiops Chromosomes/ultrastructure DNA Restriction Enzymes Genes Genes, Viral Kidney Microscopy, Electron Nucleosomes/ultrastructure Oncogene Proteins, Viral/genetics Protein Kinases/genetics Simian virus 40/enzymology,genetics
Chemicals
Antigens, Polyomavirus Transforming Antigens, Viral, Tumor Nucleosomes Oncogene Proteins, Viral Protein Kinases DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Weiss E
Ghose D
Schultz P
Oudet P
References (44)
44 references, click to expand
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00327472
Published
1985-00-00
Pages
391-400
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00327472 View journal details
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