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PMID: 2994966 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Three related centromere proteins are absent from the inactive centromere of a stable isodicentric chromosome.

Chromosoma ·Vol. 92 ·No. 4 ·1985-00-00 ·Pages 290-6

Earnshaw WC, Migeon BR

Abstract

We developed an aqueous spreading procedure that permits simultaneous analysis of human chromosomes by Q-banding and indirect immunofluorescence. Using this methodology and anticentromere antibodies from an autoimmune patient we compared the active and inactive centromeres of an isodicentric X chromosome. We show that a family of structurally related human centromere proteins (CENP-A, CENP-B, and CENP-C) is detectable only at the active centromere. These antigens therefore may be regarded both as morphological and functional markers for active centromeres.

MeSH Terms
Cell Transformation, Viral Centromere/analysis,ultrastructure Chromosome Banding Chromosomes/ultrastructure Female Herpesvirus 4, Human/genetics Humans Karyotyping Lymphocytes/cytology Metaphase X Chromosome/ultrastructure
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Earnshaw W C
Migeon B R
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49 references, click to expand
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00329812
Published
1985-00-00
Pages
290-6
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
Grants
NIGMS NIH HHS · GM-30985 · United States
NICHD NIH HHS · HD 05465 · United States
External Links
PubMed source DOI: 10.1007/BF00329812 View journal details
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