Sequence analysis of hepatitis A virus cDNA coding for capsid proteins and RNA polymerase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 7 ·1985-04-00 ·Pages 2143-7

Baroudy BM, Ticehurst JR, Miele TA, Maizel JV, Purcell RH, Feinstone SM

Abstract

We report here the nucleotide sequence corresponding to two large regions of the hepatitis A virus (HAV) genome. These comprise a sequence of 3274 bases corresponding to the 5' end of the genome, which includes the putative capsid protein region of this picornavirus, and 1590 bases corresponding to the 3' end of the genome, terminating in a 15-base poly(A) tract. These sequences revealed that HAV had the characteristic genomic organization of picornaviruses: an open reading frame beginning approximately 750 bases from the 5' end of the RNA and a termination codon 60 bases from the 3' poly(A) tract. The predicted amino acid sequences of both regions have been compared to analogous regions previously determined for other picornaviruses. There was sufficient homology to conclude that the 5' region of HAV codes for capsid proteins and that the 3' region codes for an RNA polymerase. However, these regions of HAV were not found to be closely related to analogous regions of poliovirus, encephalomyocarditis virus, and foot and mouth disease virus.

MeSH Terms

Amino Acid Sequence Base Sequence Computers DNA-Directed RNA Polymerases/genetics Genes, Viral Hepatovirus/genetics RNA, Viral/analysis Viral Proteins/genetics Viral Structural Proteins

Chemicals

RNA, Viral Viral Proteins Viral Structural Proteins DNA-Directed RNA Polymerases

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Baroudy B M

Ticehurst J R

Miele T A

Maizel J V

Purcell R H

Feinstone S M

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24 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.82.7.2143

Published

1985-04-00

Pages

2143-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC397509

Subset

Databases

GENBANK

M14114, M14115

External Links

PubMed source DOI: 10.1073/pnas.82.7.2143 PMC Full Text View journal details

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Sequence analysis of hepatitis A virus cDNA coding for capsid proteins and RNA polymerase.

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