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PMID: 2983321 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Synthetic peptides from four separate regions of the poliovirus type 1 capsid protein VP1 induce neutralizing antibodies.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 3 ·1985-02-00 ·Pages 910-4

Chow M, Yabrov R, Bittle J, Hogle J, Baltimore D

Abstract

Peptides from different regions of the poliovirus type 1 capsid protein VP1 were synthesized. Antibodies raised against these peptides in rabbits and rats recognized the cognate peptides and denatured VP1. Peptides from four regions of VP1 generated antisera with neutralizing titers specifically against poliovirus type 1. Antisera against all other regions of VP1 failed to neutralize virus infectivity, although some of the antisera clearly bound to native virions. Thus, the neutralizing determinants on VP1 reside in specific noncontiguous regions of the protein and can be defined by specific peptides from these regions.

MeSH Terms
Amino Acid Sequence Animals Antibody Formation Antibody Specificity Peptide Fragments/immunology Poliovirus/immunology Rabbits Rats Viral Proteins/immunology Viral Structural Proteins
Chemicals
Peptide Fragments Viral Proteins Viral Structural Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chow M
Yabrov R
Bittle J
Hogle J
Baltimore D
References (29)
29 references, click to expand
  1. Human-mouse hybrid cell lines and susceptibility to poliovirus. I. Conversion from polio sensitivity to polio resistance accompanying loss of human gene-dependent polio receptors.
    J Virol. 1970 Jun;5(6):677-81 PMID: 4317112
  2. The mammalian cell-virus relationship. IV. Infection of naturally insusceptible cells with enterovirus ribonucleic acid.
    J Exp Med. 1959 Jul 1;110(1):65-80 PMID: 13664869
  3. Investigation of the structure of polio- and human rhinovirions through the use of selective chemical reactivity.
    Virology. 1976 May;71(1):207-16 PMID: 179200
  4. New procedures for preparation and isolation of conjugates of proteins and a synthetic copolymer of D-amino acids and immunochemical characterization of such conjugates.
    Biochemistry. 1979 Feb 20;18(4):690-3 PMID: 84683
  5. Enhancement of immune cellular agglutination by use of an avidin-biotin system.
    Clin Chem. 1979 Sep;25(9):1572-80 PMID: 572747
  6. Topographical studies on poliovirus capsid proteins by chemical modification and cross-linking with bifunctional reagents.
    J Gen Virol. 1979 Aug;44(2):525-34 PMID: 230294
  7. Human beta-endorphin: synthesis and characterization of analogs iodinated and tritiated at tyrosine residues 1 and 27.
    Int J Pept Protein Res. 1980 Oct;16(4):311-20 PMID: 7461911
  8. Primary structure, gene organization and polypeptide expression of poliovirus RNA.
    Nature. 1981 Jun 18;291(5816):547-53 PMID: 6264310
  9. A neutralizing monoclonal antibody against poliovirus and its reaction with related antigens.
    Virology. 1981 Nov;115(1):211-5 PMID: 6270899
  10. Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4887-91 PMID: 6272282
  11. Antibodies against the chemically synthesized genome-linked protein of poliovirus react with native virus-specific proteins.
    Cell. 1982 Feb;28(2):395-404 PMID: 6174242
  12. [Structural polypeptide VP1 in poliovirus type 1 induced by neutralizing antibodies].
    C R Seances Acad Sci III. 1982 Jan 11;294(2):91-4 PMID: 6282399
  13. Monoclonal antibodies to polioviruses. Production of specific monoclonal antibodies to the Sabin vaccine strains.
    Intervirology. 1981;16(4):218-24 PMID: 6177658
  14. Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence.
    Nature. 1982 Jul 1;298(5869):30-3 PMID: 7045684
  15. Poliovirus neutralization epitopes: analysis and localization with neutralizing monoclonal antibodies.
    J Virol. 1982 Sep;43(3):997-1005 PMID: 6183443
  16. Preliminary studies of crystals of poliovirus type I.
    J Mol Biol. 1982 Oct 5;160(4):663-8 PMID: 6294308
  17. Isolated poliovirus capsid protein VP1 induces a neutralizing response in rats.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7518-21 PMID: 6296837
  18. Location and primary structure of a major antigenic site for poliovirus neutralization.
    Nature. 1983 Feb 24;301(5902):674-9 PMID: 6186919
  19. Detection by monoclonal antibodies of an antigenic determinant critical for poliovirus neutralization present on VP1 and on heat-inactivated virions.
    Virology. 1983 Apr 30;126(2):707-10 PMID: 6190311
  20. Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):4045-9 PMID: 6191328
  21. Neutralization of poliovirus by a monoclonal antibody: kinetics and stoichiometry.
    Virology. 1983 Jun;127(2):412-25 PMID: 6306918
  22. Critical role of an eight-amino acid sequence of VP1 in neutralization of poliovirus type 3.
    Nature. 1983 Aug 4-10;304(5925):459-62 PMID: 6192344
  23. Natural variation of poliovirus neutralization epitopes.
    Infect Immun. 1983 Sep;41(3):1217-25 PMID: 6193066
  24. Priming for and induction of anti-poliovirus neutralizing antibodies by synthetic peptides.
    Nature. 1983 Aug 25-31;304(5928):699-703 PMID: 6310403
  25. Bivalent attachment of antibody onto poliovirus leads to conformational alteration and neutralization.
    J Virol. 1983 Nov;48(2):547-50 PMID: 6194315
  26. A poliovirus type 1 neutralization epitope is located within amino acid residues 93 to 104 of viral capsid polypeptide VP1.
    EMBO J. 1983;2(11):2019-24 PMID: 6196195
  27. Antibodies against a preselected peptide recognize and neutralize foot and mouth disease virus.
    EMBO J. 1982;1(7):869-74 PMID: 6203738
  28. The mammalian cell-virus relationship. II. Adsorption, reception, and eclipse of poliovirus by HeLa cells.
    J Exp Med. 1959 May 1;109(5):487-504 PMID: 13641572
  29. Chemical synthesis of peptides and proteins.
    Annu Rev Biochem. 1970;39:841-66 PMID: 4920831
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.3.910
Published
1985-02-00
Pages
910-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397157
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.3.910 PMC Full Text View journal details
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