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PMID: 2983084 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Recombination between a defective retrovirus and homologous sequences in host DNA: reversion by patch repair.

Journal of virology ·Vol. 53 ·No. 3 ·1985-03-00 ·Pages 719-26

Schwartzberg P, Colicelli J, Goff SP

Abstract

The genomes of mammalian species contain multiple copies of sequences homologous to exogenous retroviruses. When a mutant retrovirus carrying a lethal deletion in an essential viral gene was introduced into mammalian cells, revertant viruses appeared and spread throughout the culture. Analysis of one such revertant showed that the mutation had been repaired by homologous recombination with endogenous sequences. Our results suggest that defective retroviruses can draw upon the genetic complement of the host cell to repair lesions in viral genes.

MeSH Terms
Animals Base Sequence Cell Line Chromosome Deletion Cloning, Molecular DNA, Viral/analysis Defective Viruses/genetics Genes, Viral Leukemia Virus, Murine/genetics Mice Mutation Recombination, Genetic Retroviridae/genetics
Chemicals
DNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schwartzberg P
Colicelli J
Goff S P
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50 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.53.3.719-726.1985
Published
1985-03-00
Pages
719-26
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254699
Subset
IM
Grants
NCI NIH HHS · CA 30488 · United States
Databases
GENBANK
K03364, M17738
External Links
PubMed source DOI: 10.1128/JVI.53.3.719-726.1985 PMC Full Text View journal details
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