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PMID: 6601726 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Deletion mutants of Moloney murine leukemia virus which lack glycosylated gag protein are replication competent.

Journal of virology ·Vol. 46 ·No. 2 ·1983-05-00 ·Pages 538-46

Schwartzberg P, Colicelli J, Goff SP

Abstract

A series of deletion mutations localized near the 5' end of the Moloney murine leukemia virus genome was generated by site-specific mutagenesis of cloned viral DNA. The mutants recovered from such deleted DNAs failed to synthesize the normal glycosylated gag protein gPr80gag. Two of the mutants made no detectable protein, and a third mutant, containing a 66-base pair deletion, synthesized an altered gag protein which was not glycosylated. All the mutants made normal amounts of the internal Pr65gag protein. The viruses were XC positive and replicated normally in NIH/3T3 cells as well as in lymphoid cell lines. These results indicate that the additional peptides of the glycosylated gag protein are encoded near the 5' end, that the glycosylated and internal gag proteins are synthesized independently, and that the glycosylated gag protein is not required during the normal replication cycle. In addition, the region deleted in these mutants apparently encodes no cis-acting function needed for replication. Thus, all essential sequences, including those for packaging viral RNA, must lie outside this area.

MeSH Terms
Animals Cell Line Cloning, Molecular Gene Products, gag Genes, Viral Lymphocytes/microbiology Mice Moloney murine leukemia virus/genetics,physiology Mutation Viral Proteins/analysis,genetics,physiology Virus Replication
Chemicals
Gene Products, gag Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schwartzberg P
Colicelli J
Goff S P
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40 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.46.2.538-546.1983
Published
1983-05-00
Pages
538-46
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255156
Subset
IM
Grants
NCI NIH HHS · 5 RO1 CA-30488 · United States
Databases
GENBANK
J02255, J02256, J02257
External Links
PubMed source DOI: 10.1128/JVI.46.2.538-546.1983 PMC Full Text View journal details
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