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PMID: 6997511 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequence relationship of glycosylated and unglycosylated gag polyproteins of Moloney murine leukemia virus.

Journal of virology ·Vol. 35 ·No. 1 ·1980-07-00 ·Pages 41-51

Edwards SA, Fan H

Abstract

Both glycosylated and unglycosylated polyproteins coded by the gag gene are produced in cells infected with Moloney murine leukemia virus. GpP80gag is a glycosylated precursor of a larger gag glycoprotein exported to the cell surface, whereas Pr65gag is an unglycosylated precursor of the virion internal structural proteins. GpP80gag contains not only carbohydrate, but also additional polypeptide sequences not found in Pr65gag. In the experiment reported here, we localized the differences between GpP80gag and Pr65gag with respect to the domains of the individual gag proteins. This was done by comparison of partial proteolytic cleavage fragments from Pr65gag, from GpP80gag, and from the unglycosylated form of GpP80gag (P75gag) which had been immunoprecipitated by antisera specific for gag proteins p30, p15, and p10. We conclude that the additional polypeptide sequences in GpP80gag are located at or very near the amino terminus of the polyprotein. The carbohydrate in GpP80gag is attached to polypeptide sequences held in common between GpP80gag and Pr65gag.

MeSH Terms
Electrophoresis, Polyacrylamide Gel Genes Glycoproteins/biosynthesis Mannose/metabolism Molecular Weight Moloney murine leukemia virus/genetics Peptide Chain Initiation, Translational Peptide Fragments Peptide Hydrolases/pharmacology Protein Precursors/genetics Viral Proteins/biosynthesis,genetics
Chemicals
Glycoproteins Peptide Fragments Protein Precursors Viral Proteins Peptide Hydrolases Mannose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Edwards S A
Fan H
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31 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.35.1.41-51.1980
Published
1980-07-00
Pages
41-51
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC288781
Subset
IM
Grants
NCI NIH HHS · 5T01-CA-15274 · United States
NCI NIH HHS · CA-14195 · United States
NCI NIH HHS · CA-15747 · United States
External Links
PubMed source DOI: 10.1128/JVI.35.1.41-51.1980 PMC Full Text View journal details
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