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PMID: 291002 Published · ppublish English Journal Article

In vitro core particle and nucleosome assembly at physiological ionic strength.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 7 ·1979-07-00 ·Pages 3284-8

Ruiz-Carrillo A, Jorcano JL, Eder G, Lurz R

Abstract

Nucleosome core particles have been efficiently assembled in vitro by direct interaction of histones and DNA at physiological ionic strength, as assayed by digestion with DNases, supercoiling of relaxed circular DNA, and electron microscopy. Reconstitution was achieved either by the simultaneous addition of all core histones, or by the sequential binding of H3 . H4 tetramer and H2A . H2B dimer to DNA. Micrococcal nuclease digestion and electron microscopy studies indicated that there is heterogeneity in the spacings at which core particles are assembly on the DNA. Length measurements of oligomeric DNA produced during the course of the digestion suggest that the core histone octamer can organize 167 (+/- 4) rather than 145 base pairs of DNA, the extra 20 base pairs being quickly digested. Binding of histone H1 to core particles resulted in the protection of about 165 base pairs of DNA from nuclease attack. Because the core histone octamer is fully dissociated into H3 . H4 tetramer and H2A . H2B dimer at physiological ionic strength, our results would suggest that in vivo core particle assembly may also occur by interaction of these two complexes on the nascent DNA.

MeSH Terms
Animals Chickens Chromatin/metabolism DNA/metabolism Erythrocytes/metabolism Histones/metabolism In Vitro Techniques Nucleic Acid Conformation Nucleosomes/metabolism Osmolar Concentration Protein Conformation
Chemicals
Chromatin Histones Nucleosomes DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ruiz-Carrillo A
Jorcano J L
Eder G
Lurz R
References (35)
35 references, click to expand
  1. H3.H4 tetramer directs DNA and core histone octamer assembly in the nucleosome core particle.
    Biochemistry. 1979 Mar 6;18(5):768-74 PMID: 217424
  2. DNA supercoiling by Xenopus laevis oocyte extracts: requirement for a nuclear factor.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4873-6 PMID: 217004
  3. Stability of nucleosomes in native and reconstituted chromatins.
    Nucleic Acids Res. 1976 Nov;3(11):3173-92 PMID: 188018
  4. Assembly of SV40 chromatin in a cell-free system from Xenopus eggs.
    Cell. 1977 Feb;10(2):237-43 PMID: 189936
  5. DNA-DNA gyrase complex: the wrapping of the DNA duplex outside the enzyme.
    Cell. 1978 Nov;15(3):979-84 PMID: 153201
  6. Assembly of newly replicated chromatin.
    Cell. 1978 Nov;15(3):969-77 PMID: 103629
  7. Chromatin.
    Nature. 1978 Jan 12;271(5641):115-22 PMID: 340956
  8. Structure of chromatin.
    Annu Rev Biochem. 1977;46:931-54 PMID: 332067
  9. Supercoiling energy and nucleosome formation: the role of the arginine-rich histone kernel.
    Nucleic Acids Res. 1977;4(5):1159-81 PMID: 331250
  10. Compact oligomers and nucleosome phasing.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3583-7 PMID: 278974
  11. Nucleohistone assembly: sequential binding of histone H3-H4 tetramer and histone H2A-H2B dimer to DNA.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 1:165-70 PMID: 277338
  12. Small angle neutron scattering studies of chromatin subunits in solution.
    Cell. 1977 Jan;10(1):139-51 PMID: 556978
  13. The structure of the chromatin core particle in solution.
    Nucleic Acids Res. 1977 Sep;4(9):3199-214 PMID: 561952
  14. An octamer of core histones in solution: central role of the H3-H4 tetramer in the self-assembly.
    Biochemistry. 1979 Mar 6;18(5):760-8 PMID: 420814
  15. Structure of the chromatosome, a chromatin particle containing 160 base pairs of DNA and all the histones.
    Biochemistry. 1978 Dec 12;17(25):5524-31 PMID: 728412
  16. The histone core complex: an octamer assembled by two sets of protein-protein interactions.
    Biochemistry. 1978 Nov 14;17(23):4955-64 PMID: 718868
  17. Physical studies of nucleosome assemble.
    Biochemistry. 1978 Oct 17;17(21):4533-40 PMID: 718856
  18. Nucleosomes are assembled by an acidic protein which binds histones and transfers them to DNA.
    Nature. 1978 Oct 5;275(5679):416-20 PMID: 692721
  19. DNA condensation with polyamines. II. Electron microscopic studies.
    J Mol Biol. 1978 May 25;121(3):327-37 PMID: 671541
  20. Closely spaced nucleosome cores in reconstituted histone.DNA complexes and histone-H1-depleted chromatin.
    Eur J Biochem. 1978 Feb;83(2):615-28 PMID: 631138
  21. Isolation and characterization of a spacerless dinucleosome from H1-deleted chromatin.
    Nucleic Acids Res. 1977 Dec;4(12):4077-89 PMID: 600791
  22. Characterization of the octamer of histones free in solution.
    J Mol Biol. 1977 Nov;116(4):769-81 PMID: 592400
  23. Structure of nucleosome core particles of chromatin.
    Nature. 1977 Sep 1;269(5623):29-36 PMID: 895884
  24. Action of micrococcal nuclease on chromatin and the location of histone H1.
    J Mol Biol. 1977 Jan 25;109(3):393-404 PMID: 833849
  25. Chromatin nu bodies: isolation, subfractionation and physical characterization.
    Nucleic Acids Res. 1976 Dec;3(12):3271-91 PMID: 1005117
  26. Proton magnetic resonance ttudies of the interactions of histones F1 and F2B with DNA.
    Nat New Biol. 1971 Feb 3;229(5):149-50 PMID: 5280096
  27. A histone cross-complexing pattern.
    Biochemistry. 1974 Nov 19;13(24):4992-7 PMID: 4474009
  28. Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):4876-80 PMID: 174079
  29. A two-subunit histone complex from calf thymus.
    Biochem Biophys Res Commun. 1974 Jul 24;59(2):542-7 PMID: 4859197
  30. Chromatin structure; oligomers of the histones.
    Science. 1974 May 24;184(4139):865-8 PMID: 4825888
  31. Isolation of a histone IIb1-IIb2 complex.
    Biochem Biophys Res Commun. 1973 Oct 15;54(4):1588-94 PMID: 4754727
  32. Linking numbers and nucleosomes.
    Proc Natl Acad Sci U S A. 1976 Aug;73(8):2639-43 PMID: 1066673
  33. The organization of histones and DNA in chromatin: evidence for an arginine-rich histone kernel.
    Cell. 1976 Jul;8(3):333-47 PMID: 986252
  34. Processing of newly synthesized histone molecules.
    Science. 1975 Oct 10;190(4210):117-28 PMID: 1166303
  35. Internal structure of the chromatin subunit.
    Nucleic Acids Res. 1974 Nov;1(11):1573-8 PMID: 10793712
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.7.3284
Published
1979-07-00
Pages
3284-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383809
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.7.3284 PMC Full Text View journal details
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