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PMID: 217004 Published · ppublish English Journal Article

DNA supercoiling by Xenopus laevis oocyte extracts: requirement for a nuclear factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 10 ·1978-10-00 ·Pages 4873-6

Baldi MI, Mattoccia E, Tocchini-Valentini GP

Abstract

A purified system is described for the introduction of negative supercoils into simian virus 40 DNA. The system consists of histones H2A, H2B, H3, and H4, DNA-relaxing enzyme, and a purified factor from Xenopus laevis stage 6 oocyte nuclei. The nuclei are prepared en masse by the technique of F. Scalenghe, M. Buscaglia, C. Steinheil, and M. Crippa [(1978) Chromosoma 60, 299-308]. The supercoiled simian virus 40 DNA prepared by this method is indistinguishable from simian virus 40 supercoiled DNA prepared from infected monkey cells.

MeSH Terms
Animals Cell Nucleus DNA Topoisomerases, Type I DNA, Superhelical DNA, Viral/genetics Female Histones Oocytes Simian virus 40/genetics Xenopus/genetics
Chemicals
DNA, Superhelical DNA, Viral Histones DNA Topoisomerases, Type I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Baldi M I
Mattoccia E
Tocchini-Valentini G P
References (17)
17 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.10.4873
Published
1978-10-00
Pages
4873-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC336223
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.10.4873 PMC Full Text View journal details
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