Large scale isolation of nuclei and nucleoli from vitellogenic oocytes of Xenopus laevis.

Chromosoma ·Vol. 66 ·No. 4 ·1978-05-16 ·Pages 299-308

Scalenghe F, Buscaglia M, Steinheil C, Crippa M

Abstract

A new method is described which allows the purification of large quantities of nuclei (germinal vesicles) from Xenopus laevis vitellogenic oocytes of different developmental stages. From the isolated nuclei, purified nucleoli were obtained. The isolated germinal vesicles are transcriptionally active showing endogenous RNA polymerase activity as well as a high level of activity with an exogenously added template.

MeSH Terms

Animals Cell Fractionation/methods Cell Nucleolus/metabolism Cell Nucleus/metabolism DNA/metabolism DNA-Directed RNA Polymerases/metabolism Female Nucleic Acid Hybridization Oocytes/metabolism RNA/genetics,metabolism Transcription, Genetic Xenopus

Chemicals

RNA DNA DNA-Directed RNA Polymerases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Scalenghe F

Buscaglia M

Steinheil C

Crippa M

References (13)

13 references, click to expand

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Purified DNAs are transcribed after microinjection into Xenopus oocytes.
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1502-6 PMID: 193103
Persistence in early amphibian embryos of informational RNA's from the lampbrush chromosome stage of oögenesis.
Proc Natl Acad Sci U S A. 1967 Apr;57(4):885-92 PMID: 5232368
Coupled transcription-translation of DNA injected into Xenopus oocytes.
Cell. 1977 Sep;12(1):175-82 PMID: 198138
Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA.
Science. 1968 Apr 19;160(3825):272-80 PMID: 4867987
Localization of amplified ribosomal DNA in the oocyte of Xenopus laevis.
Biochim Biophys Acta. 1968 Aug 23;166(1):274-6 PMID: 5698061
Genetic information in oocyte RNA.
J Mol Biol. 1971 Mar 28;56(3):491-506 PMID: 5573011
High-fidelity transcription of 5S DNA injected into Xenopus oocytes.
Proc Natl Acad Sci U S A. 1977 May;74(5):2064-8 PMID: 266727
Defined nutrient medium for the in vitro maintenance of Xenopus laevis oocytes.
In Vitro. 1976 Jun;12(6):418-27 PMID: 986365
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Dev Biol. 1977 Feb;55(2):375-86 PMID: 557006
[Biochemical studies on oogenesis. I. RNA synthesis and accumulation during oogenesis of the South African toad Xenopus laevis].
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Article Info

Journal

Chromosoma

Abbr.

Chromosoma

ISSN

0009-5915

DOI

10.1007/BF00328531

Published

1978-05-16

Pages

299-308

Language

English

Region

Austria

NLM ID

2985138R

Subset

External Links

PubMed source DOI: 10.1007/BF00328531 View journal details

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Large scale isolation of nuclei and nucleoli from vitellogenic oocytes of Xenopus laevis.

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