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PMID: 2879222 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Multiple transcripts from the Antennapedia gene of Drosophila melanogaster.

Molecular and cellular biology ·Vol. 6 ·No. 12 ·1986-12-00 ·Pages 4667-75

Stroeher VL, Jorgensen EM, Garber RL

Abstract

The structures of four major transcripts from the homeotic gene Antennapedia of Drosophila melanogaster were determined. These transcripts constitute two RNA classes, each class initiating from a unique promoter but sharing 3' exons. Within the shared sequences is a major open reading frame encoding a 378-amino-acid protein as well as alternative polyadenylation sites. Although the RNA classes differ in their 5' sequences, both leaders contain many AUGs upstream of the major open reading frame. For the two RNA classes, neither gross tissue nor temporal specificity was observed. However, the second poly(A) site is preferred in neural tissue. The structural diversity of the RNAs is discussed in relation to biological functions of the Antennapedia locus.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA/isolation & purification Drosophila melanogaster/genetics Embryo, Nonmammalian Endonucleases Genes Genes, Homeobox Insect Hormones/genetics Pupa Ribonucleases Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic
Chemicals
Insect Hormones DNA Endonucleases Ribonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stroeher V L
Jorgensen E M
Garber R L
References (56)
56 references, click to expand
  1. opa: a novel family of transcribed repeats shared by the Notch locus and other developmentally regulated loci in D. melanogaster.
    Cell. 1985 Jan;40(1):55-62 PMID: 2981631
  2. Multiple upstream AUG codons mediate translational control of GCN4.
    Cell. 1986 Apr 25;45(2):201-7 PMID: 3516411
  3. Large surface proteins of hepatitis B virus containing the pre-s sequence.
    J Virol. 1984 Nov;52(2):396-402 PMID: 6492255
  4. The preferential translation of Drosophila hsp70 mRNA requires sequences in the untranslated leader.
    Cell. 1985 Oct;42(3):903-11 PMID: 4053186
  5. Sequence of a Drosophila segmentation gene: protein structure homology with DNA-binding proteins.
    Nature. 1984 Jul 5-11;310(5972):25-31 PMID: 6330566
  6. Structural organization and sequence of the homeotic gene Antennapedia of Drosophila melanogaster.
    EMBO J. 1986 Apr;5(4):733-9 PMID: 10408949
  7. Identification of the SV40 agnogene product: a DNA binding protein.
    Nature. 1981 May 28;291(5813):346-9 PMID: 6262654
  8. The Antennapedia gene is required and expressed in parasegments 4 and 5 of the Drosophila embryo.
    EMBO J. 1986 Jan;5(1):135-141 PMID: 16453662
  9. A homoeotic mutation transforming leg to antenna in Drosophila.
    Nature. 1981 Aug 13;292(5824):635-8 PMID: 7254358
  10. Mutations affecting segment number and polarity in Drosophila.
    Nature. 1980 Oct 30;287(5785):795-801 PMID: 6776413
  11. The engrailed locus of Drosophila: structural analysis of an embryonic transcript.
    Cell. 1985 Jan;40(1):37-43 PMID: 3917855
  12. Mapping adenines, guanines, and pyrimidines in RNA.
    Nucleic Acids Res. 1977 Aug;4(8):2527-38 PMID: 409999
  13. Initiation of translation of the cauliflower mosaic virus genome from a polycistronic mRNA: evidence from deletion mutagenesis.
    EMBO J. 1984 Dec 1;3(12):2731-6 PMID: 16453575
  14. Selective translation of heat shock mRNA in Drosophila melanogaster depends on sequence information in the leader.
    EMBO J. 1985 Aug;4(8):2053-60 PMID: 2933251
  15. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.
    Cell. 1986 Jan 31;44(2):283-92 PMID: 3943125
  16. Genes controlling segmental specification in the Drosophila thorax.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7380-4 PMID: 6961417
  17. A rapid boiling method for the preparation of bacterial plasmids.
    Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
  18. Fly and frog homoeo domains show homologies with yeast mating type regulatory proteins.
    Nature. 1984 Jul 5-11;310(5972):70-1 PMID: 6429549
  19. Spatial regulation of Antennapedia and bithorax gene expression by the Polycomb locus in Drosophila.
    Cell. 1986 Mar 14;44(5):739-48 PMID: 3081265
  20. Complete nucleotide sequence of the attenuated poliovirus Sabin 1 strain genome.
    Proc Natl Acad Sci U S A. 1982 Oct;79(19):5793-7 PMID: 6310545
  21. Insertion mutagenesis to increase secondary structure within the 5' noncoding region of a eukaryotic mRNA reduces translational efficiency.
    Cell. 1985 Mar;40(3):515-26 PMID: 2982496
  22. A conserved DNA sequence in homoeotic genes of the Drosophila Antennapedia and bithorax complexes.
    Nature. 1984 Mar 29-Apr 4;308(5958):428-33 PMID: 6323992
  23. Localization of the fushi tarazu protein during Drosophila embryogenesis.
    Cell. 1985 Nov;43(1):47-57 PMID: 3000605
  24. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  25. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  26. Structure-function relationship of Rous sarcoma virus leader RNA.
    Nucleic Acids Res. 1982 Sep 11;10(17):5183-96 PMID: 6292833
  27. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.
    Gene. 1984 Jun;28(3):351-9 PMID: 6235151
  28. Analysis of larval segmentation in lethal genotypes associated with the antennapedia gene complex in Drosophila melanogaster.
    Dev Biol. 1981 Jan 15;81(1):51-64 PMID: 6780397
  29. Role of the esc+ gene product in ensuring the selective expression of segment-specific homeotic genes in Drosophila.
    J Embryol Exp Morphol. 1983 Aug;76:297-331 PMID: 6631324
  30. Regulation of Antennapedia transcript distribution by the bithorax complex in Drosophila.
    Nature. 1984 Jan 19-25;307(5948):287-9 PMID: 6420705
  31. Structural relationships among genes that control development: sequence homology between the Antennapedia, Ultrabithorax, and fushi tarazu loci of Drosophila.
    Proc Natl Acad Sci U S A. 1984 Jul;81(13):4115-9 PMID: 6330741
  32. The Drosophila developmental gene, engrailed, encodes a sequence-specific DNA binding activity.
    Nature. 1985 Dec 19-1986 Jan 1;318(6047):630-5 PMID: 4079979
  33. Spatial distribution of Antennapedia transcripts during Drosophila development.
    EMBO J. 1983;2(11):2037-46 PMID: 6416828
  34. The engrailed locus of D. melanogaster provides an essential zygotic function in precellular embryos.
    Cell. 1985 Dec;43(3 Pt 2):591-601 PMID: 3935326
  35. Homeotic genes, the homeo box, and the genetic control of development.
    Cold Spring Harb Symp Quant Biol. 1985;50:243-51 PMID: 3868480
  36. Reversible inhibition of translation by Xenopus oocyte-specific proteins.
    Nature. 1984 May 24-30;309(5966):378-80 PMID: 6727992
  37. Two promoters of different strengths control the transcription of the mouse alpha-amylase gene Amy-1a in the parotid gland and the liver.
    Cell. 1983 Jun;33(2):501-8 PMID: 6190572
  38. Acceleration of nucleic acid hybridization rate by polyethylene glycol.
    Anal Biochem. 1986 Feb 1;152(2):304-7 PMID: 2421601
  39. Development of embryonic pattern in D. melanogaster as revealed by accumulation of the nuclear engrailed protein.
    Cell. 1985 Nov;43(1):59-69 PMID: 3935318
  40. Measles virus P gene codes for two proteins.
    J Virol. 1985 Mar;53(3):908-19 PMID: 3882996
  41. Common properties of proteins encoded by the Antennapedia complex genes of Drosophila melanogaster.
    Cold Spring Harb Symp Quant Biol. 1985;50:253-62 PMID: 3938363
  42. The molecular organization of the Antennapedia locus of Drosophila.
    Cell. 1983 Dec;35(3 Pt 2):763-76 PMID: 6418389
  43. Hybridization of denatured RNA transferred or dotted nitrocellulose paper.
    Methods Enzymol. 1983;100:255-66 PMID: 6194404
  44. Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.
    Microbiol Rev. 1983 Mar;47(1):1-45 PMID: 6343825
  45. Attenuation in the control of SV40 gene expression.
    Cell. 1982 May;29(1):183-93 PMID: 6286139
  46. Protein products of the bithorax complex in Drosophila.
    Cell. 1984 Nov;39(1):163-71 PMID: 6091908
  47. Segmental distribution of bithorax complex proteins during Drosophila development.
    Nature. 1985 Feb 14-20;313(6003):545-51 PMID: 3918274
  48. Plasmid screening at high colony density.
    Gene. 1980 Jun;10(1):63-7 PMID: 6997135
  49. Genomic and cDNA clones of the homeotic locus Antennapedia in Drosophila.
    EMBO J. 1983;2(11):2027-36 PMID: 6416827
  50. Sequence, structure, and codon preference of the Drosophila ribosomal protein 49 gene.
    Nucleic Acids Res. 1984 Jul 11;12(13):5495-513 PMID: 6087289
  51. Identification of two distinct regulatory regions adjacent to the human beta-interferon gene.
    Cell. 1983 Oct;34(3):865-79 PMID: 6313211
  52. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  53. Homeo box genes of the Antennapedia and bithorax complexes of Drosophila.
    Cell. 1985 Nov;43(1):71-80 PMID: 2416463
  54. Cloning exons of mapping of transcription: characterization of the Drosophila melanogaster alcohol dehydrogenase gene.
    Nucleic Acids Res. 1983 Jul 25;11(14):4735-52 PMID: 6410356
  55. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  56. Nucleic acid hybridization to the DNA of cytological preparations.
    Methods Cell Biol. 1975;10:1-16 PMID: 1102840
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.12.4667-4675.1986
Published
1986-12-00
Pages
4667-75
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367252
Subset
IM
Grants
NIGMS NIH HHS · GM33219 · United States
NCRR NIH HHS · RR-07096 · United States
Databases
GENBANK
M14699, M14700, M14701, M20704, M20705
External Links
PubMed source DOI: 10.1128/mcb.6.12.4667-4675.1986 PMC Full Text View journal details
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