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PMID: 2860636 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

An in vitro system for accurate transcription initiation of chloroplast protein genes.

Nucleic acids research ·Vol. 13 ·No. 4 ·1985-02-25 ·Pages 1283-302

Orozco EM, Mullet JE, Chua NH

Abstract

We have developed an homologous in vitro system from spinach chloroplasts that correctly initiates transcription of the plastid genes for the large subunit of ribulose-1,5-bisphosphate carboxylase (rbcL) and the beta subunit of the plastid ATPase (atpB). The transcriptionally active extracts from spinach chloroplasts require circular DNA templates for specific initiation. The RNA polymerase activity is insensitive to rifampicin. The extent of transcription in vitro is a function of the extract:template ratio. The efficiency of the rbcL transcription in vitro is more than one transcript per one hundred templates per hour.

MeSH Terms
Cell-Free System Chloroplasts/physiology DNA, Single-Stranded/genetics DNA-Directed RNA Polymerases/antagonists & inhibitors Plant Proteins/genetics Plants/genetics Proton-Translocating ATPases/genetics Ribulose-Bisphosphate Carboxylase/genetics Rifampin/pharmacology Templates, Genetic Transcription, Genetic
Chemicals
DNA, Single-Stranded Plant Proteins DNA-Directed RNA Polymerases Proton-Translocating ATPases Ribulose-Bisphosphate Carboxylase Rifampin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Orozco E M
Mullet J E
Chua N H
References (23)
23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.4.1283
Published
1985-02-25
Pages
1283-302
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341072
Subset
IM
Grants
NIGMS NIH HHS · GM08114 · United States
NIGMS NIH HHS · GM30726 · United States
External Links
PubMed source DOI: 10.1093/nar/13.4.1283 PMC Full Text View journal details
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