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PMID: 6638510 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Spectrophotometric quantitation of silver grains eluted from autoradiograms.

Analytical biochemistry ·Vol. 133 ·No. 2 ·1983-09-00 ·Pages 511-4

Suissa M

Abstract

Autoradiograms or fluorograms can be quantified by eluting the silver grains from the developed film with 1 M NaOH and measuring the absorbance of the eluate in a conventional spectrometer. In contrast to direct scanning of the film, the present method is rapid, requires only simple equipment, and permits accurate quantitation of even heavily exposed bands.

MeSH Terms
Autoradiography/methods Fluorescence Silver Spectrophotometry/methods
Chemicals
Silver
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Suissa M
Article Info
Journal
Analytical biochemistry
Abbr.
Anal Biochem
ISSN
0003-2697
DOI
10.1016/0003-2697(83)90117-3
Published
1983-09-00
Pages
511-4
Language
English
Region
United States
NLM ID
0370535
Subset
IM
External Links
PubMed source DOI: 10.1016/0003-2697(83)90117-3 View journal details
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