Structures of the genes for the beta and epsilon subunits of spinach chloroplast ATPase indicate a dicistronic mRNA and an overlapping translation stop/start signal.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 20 ·1982-10-00 ·Pages 6260-4

Zurawski G, Bottomley W, Whitfeld PR

Abstract

A 2,4-kilobase-pair region of spinach chloroplast DNA adjacent to the gene for the large subunit of ribulosebisphosphate carboxylase has been analyzed by RNA hybridization, in vitro transcription/translation, and DNA sequence determination. The analysis indicates that this region carries the genes for the beta and epsilon subunits of chloroplast ATPase and that the two genes are cotranscribed into a dicistronic mRNA with 4-base-pair overlap between the stop codon of the beta-subunit gene and the start codon of the epsilon-subunit gene. The ATPase and carboxylase genes are transcribed divergently with respect to each other. The deduced amino acid sequences of the beta and epsilon subunits from spinach show 67% and 26% homology, respectively, with the published sequences of the beta and epsilon subunits of Escherichia coli ATPase.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Zurawski G

Division of Plant Industry, Commonwealth Scientific and Industrial Research Organisation, Post Office Box 1600, Canberra City, A.C.T. 2601, Australia.

Bottomley W

Whitfeld P R

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.79.20.6260

Published

1982-10-00

Pages

6260-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC347100

External Links

PubMed source DOI: 10.1073/pnas.79.20.6260 PMC Full Text View journal details

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Structures of the genes for the beta and epsilon subunits of spinach chloroplast ATPase indicate a dicistronic mRNA and an overlapping translation stop/start signal.

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