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PMID: 2850487 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of an SNR gene locus in Saccharomyces cerevisiae that specifies both dispensible and essential small nuclear RNAs.

Molecular and cellular biology ·Vol. 8 ·No. 8 ·1988-08-00 ·Pages 3282-90

Zagorski J, Tollervey D, Fournier MJ

Abstract

A genetic locus is described that specifies two Saccharomyces cerevisiae small nuclear RNAs (snRNAs). The genes specifying the two snRNAs are separated by only 67 base pairs and are transcribed in the same direction. The product RNAs contain 128 and 190 nucleotides and are designated snR128 and snR190, respectively. These RNAs resemble snRNAs of other eucaryotes in nuclear localization and possession of a 5' trimethylguanosine cap. Neither snRNA is related in sequence to previously described vertebrate or yeast snRNAs. Both RNAs exhibit properties consistent with nucleolar organization and hydrogen bonding to pre-rRNA species, suggesting possible roles in ribosome biogenesis. The snR128 species cosediments with deproteinized 27S pre-rRNA, whereas snR190 is associated with a 20S intermediate. Gene disruption in vitro followed by replacement of the chromosomal alleles reveals that SNR128 is essential, whereas SNR190 is not.

MeSH Terms
Base Sequence DNA Restriction Enzymes Genes, Fungal Molecular Sequence Data Nucleic Acid Hybridization Nucleotide Mapping RNA Caps/genetics RNA, Small Nuclear/genetics Saccharomyces cerevisiae/genetics,growth & development Transcription, Genetic
Chemicals
RNA Caps RNA, Small Nuclear DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zagorski J
Department of Biochemistry, University of Massachusetts, Amherst 01003.
Tollervey D
Fournier M J
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48 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.8.3282-3290.1988
Published
1988-08-00
Pages
3282-90
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363561
Subset
IM
Grants
NIGMS NIH HHS · GM19351 · United States
Databases
GENBANK
M21124
External Links
PubMed source DOI: 10.1128/mcb.8.8.3282-3290.1988 PMC Full Text View journal details
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