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PMID: 2933810 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The 3' splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein.

Science (New York, N.Y.) ·Vol. 230 ·No. 4732 ·1985-12-20 ·Pages 1344-9

Chabot B, Black DL, LeMaster DM, Steitz JA

Abstract

A component present in splicing extracts selectively binds the 3' splice site of a precursor messenger RNA (pre-mRNA) transcript of a human beta-globin gene. Since this component can be immunoprecipitated by either autoantibodies of the Sm class or antibodies specifically directed against trimethylguanosine, it is a small nuclear ribonucleoprotein (snRNP). Its interaction with the 3' splice site occurs rapidly even at 0 degrees C, does not require adenosine triphosphate, and is altered by certain mutations in the 3' splice site region. Binding is surprisingly insensitive to treatment of the extract with micrococcal nuclease. The U5 particle is the only abundant Sm snRNP with a capped 5' end that is equally resistant to micrococcal nuclease. This suggests that, in addition to the U1 and U2 snRNP's, U5 snRNP's participate in pre-mRNA splicing.

MeSH Terms

Base Sequence Binding Sites Globins/genetics Humans Nucleic Acid Conformation Nucleic Acid Precursors/genetics,metabolism Protein Binding RNA Precursors RNA Splicing RNA, Messenger/genetics,metabolism Ribonucleoproteins/genetics,metabolism Ribonucleoproteins, Small Nuclear Transcription, Genetic

Chemicals

Nucleic Acid Precursors RNA Precursors RNA, Messenger Ribonucleoproteins Ribonucleoproteins, Small Nuclear Globins

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Chabot B

Black D L

LeMaster D M

Steitz J A

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.2933810

Published

1985-12-20

Pages

1344-9

Language

English

Region

United States

NLM ID

0404511

Subset

Grants

NIGMS NIH HHS · GM-26154 · United States

Databases

GENBANK

J00093, J00094, J00096, J00158, J00159, J00160, J00161, J00162, J00163, J00164, J00165, J00166, J00167, J00168, J00169, J00170, J00171, J00172, J00173, J00174, J00175, J00177, J00178, J00179, K01239, K01890, K02544, M18047, M19067, X00423

External Links

PubMed source DOI: 10.1126/science.2933810 View journal details

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The 3' splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein.

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