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PMID: 3534883 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Small nuclear RNAs from Saccharomyces cerevisiae: unexpected diversity in abundance, size, and molecular complexity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 21 ·1986-11-00 ·Pages 8097-101

Riedel N, Wise JA, Swerdlow H, Mak A, Guthrie C

Abstract

Previous work showed that the simple eukaryote Saccharomyces cerevisiae contains a group of RNAs with the general structural properties predicted for small nuclear RNAs (snRNAs), including possession of the characteristic trimethylguanosine 5'-terminal cap. It was also demonstrated that, unlike their metazoan counterparts, the yeast snRNAs are present in low abundance (200-500 molecules per haploid cell). We have now used antibody directed against the 5' cap to investigate the total set size of snRNAs in this organism. We present evidence that the number of distinct yeast snRNAs is on the order of several dozen, that the length of the capped RNAs can exceed 1000 nucleotides, and that the relative abundance of a subset of these RNAs is 1/5th to 1/20th that of the class of snRNAs described previously. These findings suggest that the six highly abundant species of snRNAs (U1-U6) typically reported in metazoans may represent a serious underestimation of the total diversity of snRNAs in eukaryotes.

MeSH Terms

Cloning, Molecular DNA/analysis Nucleic Acid Hybridization RNA, Small Nuclear/analysis,genetics,immunology Saccharomyces cerevisiae/genetics

Chemicals

RNA, Small Nuclear DNA

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Riedel N

Wise J A

Swerdlow H

Mak A

Guthrie C

References (26)

26 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.83.21.8097

Published

1986-11-00

Pages

8097-101

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC386874

Subset

Grants

NIGMS NIH HHS · GM07618 · United States

NIGMS NIH HHS · GM21119 · United States

External Links

PubMed source DOI: 10.1073/pnas.83.21.8097 PMC Full Text View journal details

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Small nuclear RNAs from Saccharomyces cerevisiae: unexpected diversity in abundance, size, and molecular complexity.

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