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PMID: 2845956 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

D-myo-inositol 1,4,5-trisphosphate phosphatase in skeletal muscle.

The Biochemical journal ·Vol. 254 ·No. 2 ·1988-09-01 ·Pages 525-9

Milani D, Volpe P, Pozzan T

Abstract

The presence and subcellular distribution of D-myo-inositol 1,4,5-trisphosphate phosphatase (InsP3ase) in rabbit fast-twitch skeletal muscle were investigated. A specific InsP3ase was found in both sarcotubular-membrane and soluble fractions. Membrane-bound InsP3ase accounted for 60-65% of total activity. The InsP3ase was detected both on the surface membranes and on the InsP3-sensitive intracellular Ca2+ store, i.e. the sarcoplasmic reticulum. The Km for inositol 1,4,5-trisphosphate (InsP3) ranged between 15 and 18 microM, and the highest Vmax. (19.6 nmol of InsP3 hydrolysed/min per mg of protein) was measured in a membrane fraction enriched in transverse tubules. Several known inhibitors of InsP3ase, e.g. 2,3-bisphosphoglycerate, CdCl2 and EDTA, were active on skeletal-muscle InsP3ase. Total InsP3ase activity of both rabbit and frog skeletal muscle was comparable with that of rabbit brain, liver and main pulmonary artery (smooth muscle). The present results are consistent with the hypothesis that InsP3 plays a role in excitation-contraction coupling in skeletal muscle [Volpe, Salviati, Di Virgilio & Pozzan (1985) Nature (London) 316, 347-349].

MeSH Terms
Animals Brain/enzymology Cell Membrane/enzymology In Vitro Techniques Kinetics Liver/enzymology Muscle, Smooth, Vascular/enzymology Muscles/enzymology Phosphoric Monoester Hydrolases/antagonists & inhibitors,metabolism Rabbits Sarcoplasmic Reticulum/enzymology Tissue Distribution
Chemicals
Phosphoric Monoester Hydrolases myo-inositol 1,4,5-triphosphate phosphatase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Milani D
Centro di Studio delle Biomembrane del CNR, Università di Padova, Italy.
Volpe P
Pozzan T
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31 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2540525
Published
1988-09-01
Pages
525-9
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1135109
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2540525 PMC Full Text View journal details
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