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PMID: 2994073 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Inositol 1,4,5-trisphosphate: a possible chemical link in excitation-contraction coupling in muscle.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 18 ·1985-09-00 ·Pages 6352-6

Vergara J, Tsien RY, Delay M

Abstract

The role of inositol 1,4,5-trisphosphate (InsP3) in excitation-contraction coupling in skeletal muscle was investigated by several methods. The following results were obtained. InsP3 is released by electrical stimulation of muscles. Exogenous InsP3 releases calcium from skinned muscle fibers at relatively high doses under normal conditions but does so at very low concentrations when blockers of the InsP3 5-phosphatase are present. Blockers of InsP3 release are effective blockers of calcium transients elicited by electrical stimulation of muscle fibers. It is proposed that InsP3 acts as a chemical second messenger between transverse (T)-tubular membrane depolarization and calcium release from the sarcoplasmic reticulum in skeletal muscle.

MeSH Terms
Animals Calcium/physiology Electric Stimulation In Vitro Techniques Inositol Phosphates/physiology Inositol Polyphosphate 5-Phosphatases Intracellular Membranes/physiology Membrane Potentials Muscle Contraction Muscles/physiology Phosphoric Monoester Hydrolases/metabolism Rana catesbeiana Sarcolemma/physiology Sarcoplasmic Reticulum/physiology Sugar Phosphates/physiology
Chemicals
Inositol Phosphates Sugar Phosphates Phosphoric Monoester Hydrolases Inositol Polyphosphate 5-Phosphatases Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vergara J
Tsien R Y
Delay M
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.18.6352
Published
1985-09-00
Pages
6352-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391052
Subset
IM
Grants
NIADDK NIH HHS · AM25201 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.18.6352 PMC Full Text View journal details
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