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PMID: 2940083 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Is a guanine nucleotide-binding protein involved in excitation-contraction coupling in skeletal muscle?

The EMBO journal ·Vol. 5 ·No. 2 ·1986-02-00 ·Pages 259-62

Di Virgilio F, Salviati G, Pozzan T, Volpe P

Abstract

Plasma membrane depolarization causes skeletal muscle contraction by triggering Ca2+ release from an intracellular membrane network, the sarcoplasmic reticulum. A specialized portion of the sarcoplasmic reticulum, the terminal cisternae, is junctionally associated with sarcolemmal invaginations called the transverse tubules, but the mechanism by which the action potential at the level of the transverse tubules is coupled to Ca2+ release from the terminal cisternae is still mysterious. Here we show that: (i) GTP gamma S, a non-hydrolyzable analog of GTP, elicits isometric force development in skinned muscle fibre; (ii) GTP gamma S is unable to release CA2+ from isolated sarcoplasmic reticulum fractions; (iii) the threshold for tension development is shifted to higher GTP gamma S concentrations by pre-incubation with pertussis toxin. These results suggest that a GTP-binding protein is involved in coupling the action potential of transverse tubules to Ca2+ release from the terminal cisternae.

MeSH Terms
Animals Calcium/metabolism Calcium-Transporting ATPases/metabolism Cell Membrane/physiology GTP-Binding Proteins/metabolism Guanosine 5'-O-(3-Thiotriphosphate) Guanosine Triphosphate/analogs & derivatives,pharmacology Isometric Contraction/drug effects Kinetics Muscle Contraction/drug effects Muscles/drug effects,physiology Pertussis Toxin Rabbits Sarcoplasmic Reticulum/drug effects,physiology Thionucleotides/pharmacology Virulence Factors, Bordetella/pharmacology
Chemicals
Thionucleotides Virulence Factors, Bordetella Guanosine 5'-O-(3-Thiotriphosphate) Guanosine Triphosphate Pertussis Toxin GTP-Binding Proteins Calcium-Transporting ATPases Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Di Virgilio F
Salviati G
Pozzan T
Volpe P
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35 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-02-00
Pages
259-62
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1166727
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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