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PMID: 2991913 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Inositol trisphosphate-induced calcium release and contraction in vascular smooth muscle.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 15 ·1985-08-00 ·Pages 5231-5

Somlyo AV, Bond M, Somlyo AP, Scarpa A

Abstract

Inositol 1,4,5-trisphosphate (InsP3) caused Ca release and tension development in rabbit main pulmonary artery smooth muscle permeabilized with saponin or digitonin. Both of these responses to single additions of InsP3 (0.5-30 microM) were repeatable and occurred in the presence of 0.0-1.9 mM free Mg2+. Sustained contractions were induced by InsP3. The amount of Ca released by InsP3, measured with a Ca2+-selective electrode, was also estimated to be sufficient to stimulate contraction in intact smooth muscle. Ca release was not influenced by inhibitors of mitochondrial oxidative phosphorylation. The uptake of Ca2+ from the medium into the InsP3-sensitive pool was ATP-dependent. The present results support the hypothesis that, in smooth muscle, InsP3 is the messenger, or one of the messengers, involved in transmitter-induced (pharmacomechanical) Ca release from the sarcoplasmic reticulum, which is the intracellular Ca store identified previously as the source of Ca released by norepinephrine in main pulmonary artery.

MeSH Terms
Animals Caffeine/pharmacology Calcium/metabolism Calcium-Transporting ATPases/metabolism In Vitro Techniques Male Muscle Contraction/drug effects Muscle, Smooth, Vascular/drug effects,physiology Norepinephrine/pharmacology Phosphatidylinositols/pharmacology Rabbits
Chemicals
Phosphatidylinositols Caffeine Calcium-Transporting ATPases Calcium Norepinephrine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Somlyo A V
Bond M
Somlyo A P
Scarpa A
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28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.15.5231
Published
1985-08-00
Pages
5231-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390534
Subset
IM
Grants
NHLBI NIH HHS · HL15835 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.15.5231 PMC Full Text View journal details
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