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PMID: 2839151 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Detection of inositol 1,4,5-trisphosphate kinase in retina. A direct demonstration of phosphorylation of inositol 1,4,5-trisphosphate by ATP.

The Biochemical journal ·Vol. 250 ·No. 3 ·1988-03-15 ·Pages 891-5

Tarver AP, Anderson RE

Abstract

The metabolism of myo-inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] consists of two pathways: dephosphorylation by 5-phosphomonoesterase(s) produces inositol 1,4-bisphosphate, and phosphorylation by Ins(1,4,5)P3 3-kinase yields inositol 1,3,4,5-tetrakisphosphate [Ins(1,3,4,5)P4]. The requirements for Ins(1,4,5)P3 kinase activity in retina were characterized. Apparent Km values for ATP and Ins(1,4,5)P3 are 1.4 mM and 1.3 microM respectively. A direct demonstration of phosphorylation of Ins(1,4,5)P3 by [gamma-32P]ATP was achieved. Characterization of the 32P-labelled product revealed that it had the expected chromatographic and electrophoretic properties of Ins(1,3,4,5)P4.

MeSH Terms
Adenosine Triphosphate/metabolism Animals Cattle Chromatography, High Pressure Liquid Electrophoresis, Paper In Vitro Techniques Inositol 1,4,5-Trisphosphate Inositol Phosphates/metabolism Kinetics Phosphates/metabolism Phosphorylation Phosphotransferases/metabolism Phosphotransferases (Alcohol Group Acceptor) Retina/enzymology
Chemicals
Inositol Phosphates Phosphates Inositol 1,4,5-Trisphosphate Adenosine Triphosphate Phosphotransferases Phosphotransferases (Alcohol Group Acceptor) Inositol 1,4,5-trisphosphate 3-kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tarver A P
Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030.
Anderson R E
References (30)
30 references, click to expand
  1. Isolation and separation of inositol phosphates from hydrolysates of rat tissues.
    Biochim Biophys Acta. 1965 Jun 1;98(3):574-81 PMID: 5837456
  2. Inositol 1,4,5-trisphosphate and inositol 1,2-cyclic 4,5-trisphosphate are minor components of total mass of inositol trisphosphate in thrombin-stimulated platelets. Rapid formation of inositol 1,3,4-trisphosphate.
    J Biol Chem. 1987 Dec 25;262(36):17268-71 PMID: 2826415
  3. The concentrations of free and bound magnesium in rat tissues. Relative constancy of free Mg 2+ concentrations.
    J Biol Chem. 1973 Jul 10;248(13):4811-9 PMID: 4718747
  4. The enzymatic preparation of [alpha-(32)P]nucleoside triphosphates, cyclic [32P] AMP, and cyclic [32P] GMP.
    Biochim Biophys Acta. 1979 Mar 28;562(1):11-31 PMID: 35231
  5. A volatile liquid chromatography system for nucleotides.
    Anal Biochem. 1981 Sep 15;116(2):357-60 PMID: 7316167
  6. The inositol trisphosphate phosphomonoesterase of the human erythrocyte membrane.
    Biochem J. 1982 Apr 1;203(1):169-77 PMID: 6285891
  7. Phosphodiesteratic breakdown of endogenous polyphosphoinositides in nerve ending membranes.
    Biochem Biophys Res Commun. 1983 May 16;112(3):919-26 PMID: 6303341
  8. Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositides.
    Biochem J. 1983 May 15;212(2):473-82 PMID: 6309146
  9. Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphate.
    Nature. 1983 Nov 3-9;306(5938):67-9 PMID: 6605482
  10. Inositol trisphosphate, a novel second messenger in cellular signal transduction.
    Nature. 1984 Nov 22-28;312(5992):315-21 PMID: 6095092
  11. Stepwise enzymatic dephosphorylation of inositol 1,4,5-trisphosphate to inositol in liver.
    Nature. 1984 Nov 22-28;312(5992):374-6 PMID: 6095097
  12. A direct demonstration that inositol-trisphosphate induces an increase in intracellular calcium in Limulus photoreceptors.
    Biochem Biophys Res Commun. 1984 Dec 28;125(3):1137-42 PMID: 6335035
  13. Light-induced changes in GTP and ATP in frog rod photoreceptors. Comparison with recovery of dark current and light sensitivity during dark adaptation.
    J Gen Physiol. 1985 Jan;85(1):107-21 PMID: 3968531
  14. High-performance liquid chromatographic purification of deoxynucleoside 5'-triphosphates and their use in a sensitive electrophoretic assay of misincorporation during DNA synthesis.
    J Chromatogr. 1984 Dec 28;317:283-300 PMID: 6397478
  15. Isolation of a phosphomonoesterase from human platelets that specifically hydrolyzes the 5-phosphate of inositol 1,4,5-trisphosphate.
    J Biol Chem. 1985 Jul 5;260(13):7868-74 PMID: 2989264
  16. Mass changes in myoinositol trisphosphate in human platelets stimulated by thrombin. Inhibitory effects of phorbol ester.
    J Biol Chem. 1985 Jul 25;260(15):8657-60 PMID: 3874867
  17. Rapid formation of inositol 1,3,4,5-tetrakisphosphate following muscarinic receptor stimulation of rat cerebral cortical slices.
    Biochem J. 1985 Nov 15;232(1):211-5 PMID: 4084229
  18. The inositol tris/tetrakisphosphate pathway--demonstration of Ins(1,4,5)P3 3-kinase activity in animal tissues.
    Nature. 1986 Apr 17-23;320(6063):631-4 PMID: 3010126
  19. Formation and metabolism of inositol 1,3,4,5-tetrakisphosphate in liver.
    J Biol Chem. 1986 Jun 25;261(18):8100-3 PMID: 3487541
  20. Ca2+ regulates the inositol tris/tetrakisphosphate pathway in intact and broken preparations of insulin-secreting RINm5F cells.
    J Biol Chem. 1986 Sep 15;261(26):11931-4 PMID: 3017952
  21. Thiol/disulfide redox equilibrium and kinetic behavior of chicken liver fatty acid synthase.
    J Biol Chem. 1986 Oct 5;261(28):13135-43 PMID: 3759951
  22. Rapid formation of inositol 1,3,4,5-tetrakisphosphate and inositol 1,3,4-trisphosphate in rat parotid glands may both result indirectly from receptor-stimulated release of inositol 1,4,5-trisphosphate from phosphatidylinositol 4,5-bisphosphate.
    Biochem J. 1986 Sep 1;238(2):507-16 PMID: 3026354
  23. The metabolism of tris- and tetraphosphates of inositol by 5-phosphomonoesterase and 3-kinase enzymes.
    J Biol Chem. 1987 Feb 15;262(5):2146-9 PMID: 3029066
  24. Micro-injection of inositol 1,3,4,5-tetrakisphosphate activates sea urchin eggs by a mechanism dependent on external Ca2+.
    Biochem J. 1986 Dec 15;240(3):917-20 PMID: 3827881
  25. Inositol 1,2-cyclic 4,5-trisphosphate is not a product of muscarinic receptor-stimulated phosphatidylinositol 4,5-bisphosphate hydrolysis in rat parotid glands.
    Biochem J. 1987 Apr 1;243(1):211-8 PMID: 3038079
  26. Inositol(1,3,4,5)tetrakisphosphate-induced activation of sea urchin eggs requires the presence of inositol trisphosphate.
    Biochem Biophys Res Commun. 1987 Jul 15;146(1):284-90 PMID: 3496884
  27. Metabolism of inositol 1,3,4-trisphosphate to a new tetrakisphosphate isomer in angiotensin-stimulated adrenal glomerulosa cells.
    J Biol Chem. 1987 Jul 25;262(21):9952-5 PMID: 3497156
  28. Binding of inositol phosphates and induction of Ca2+ release from pituitary microsomal fractions.
    Biochem J. 1987 Jun 1;244(2):493-6 PMID: 3663137
  29. Metabolism of D-myo-inositol 1,3,4,5-tetrakisphosphate by rat liver, including the synthesis of a novel isomer of myo-inositol tetrakisphosphate.
    Biochem J. 1987 Aug 15;246(1):139-47 PMID: 2823793
  30. Two dimensional then layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots.
    Lipids. 1970 May;5(5):494-6 PMID: 5483450
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2500891
Published
1988-03-15
Pages
891-5
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1148939
Subset
IM
Grants
NEI NIH HHS · EY00871 · United States
External Links
PubMed source DOI: 10.1042/bj2500891 PMC Full Text View journal details
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