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PMID: 3663137 Published · ppublish English Journal Article

Binding of inositol phosphates and induction of Ca2+ release from pituitary microsomal fractions.

The Biochemical journal ·Vol. 244 ·No. 2 ·1987-06-01 ·Pages 493-6

Spät A, Lukács GL, Eberhardt I, Kiesel L, Runnebaum B

Abstract

Bovine anterior-pituitary microsomal fractions exhibit high-affinity, saturable and reversible binding of inositol 1,4,5-[32P]trisphosphate; 50% of the labelled ligand is displaced by 3.5 nM-inositol 1,4,5-trisphosphate. 0.5 microM-inositol 1,4-bisphosphate and 10 microM-ATP. Inositol 1,4,5-trisphosphate induces the release of Ca2+ from the microsomal vesicles (half-maximal effect at 290 nM), and its action is potentiated by inositol tetrakisphosphate (half-maximal effect at 4 microM).

MeSH Terms
Animals Binding Sites Calcium/metabolism Cattle Inositol Phosphates/metabolism Microsomes/metabolism Pituitary Gland, Anterior/metabolism Sugar Phosphates/metabolism
Chemicals
Inositol Phosphates Sugar Phosphates Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Spät A
Department of Physilogy, Semmelweis University Medical School, Budapest, Hungary.
Lukács G L
Eberhardt I
Kiesel L
Runnebaum B
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2440493
Published
1987-06-01
Pages
493-6
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1148018
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2440493 PMC Full Text View journal details
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