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PMID: 6525174 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Actions of inositol phosphates on Ca2+ pools in guinea-pig hepatocytes.

The Biochemical journal ·Vol. 224 ·No. 3 ·1984-12-15 ·Pages 741-6

Burgess GM, Irvine RF, Berridge MJ, McKinney JS, Putney JW

Abstract

In permeabilized hepatocytes, inositol 1,4,5-trisphosphate, inositol 2,4,5-trisphosphate and inositol 4,5-bisphosphate induced rapid release of Ca2+ from an ATP-dependent, non-mitochondrial vesicular pool, probably endoplasmic reticulum. The order of potency was inositol 1,4,5-trisphosphate greater than inositol 2,4,5-trisphosphate greater than inositol 4,5-bisphosphate. The Ca2+-releasing action of inositol 1,4,5-trisphosphate is not inhibited by high [Ca2+], nor is it dependent on [ATP] in the range of 50 microM-1.5 mM. These results suggest a role for inositol 1,4,5-trisphosphate as a second messenger in hormone-induced Ca2+ mobilisation, and that a specific receptor is involved in the Ca2+-release mechanism.

MeSH Terms
Adenosine Triphosphate/pharmacology Animals Calcium/metabolism Cell Membrane Permeability/drug effects Dose-Response Relationship, Drug Guinea Pigs In Vitro Techniques Inositol Phosphates/pharmacology Liver/cytology,drug effects,metabolism Male Mitochondria, Liver/metabolism Saponins/pharmacology Sugar Phosphates/pharmacology Time Factors
Chemicals
Inositol Phosphates Saponins Sugar Phosphates Adenosine Triphosphate Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Burgess G M
Irvine R F
Berridge M J
McKinney J S
Putney J W
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22 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2240741
Published
1984-12-15
Pages
741-6
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1144508
Subset
IM
Grants
NIADDK NIH HHS · AM-32823 · United States
External Links
PubMed source DOI: 10.1042/bj2240741 PMC Full Text View journal details
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