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PMID: 3968531 Published · ppublish English Comparative Study Journal Article

Light-induced changes in GTP and ATP in frog rod photoreceptors. Comparison with recovery of dark current and light sensitivity during dark adaptation.

The Journal of general physiology ·Vol. 85 ·No. 1 ·1985-01-00 ·Pages 107-21

Biernbaum MS, Bownds MD

Abstract

Light decreases GTP and ATP levels in purified suspensions of physiologically active frog rod outer segments still attached to their inner segment ellipsoids (OS-IS). (a) The GTP decrease is slower in OS-IS (t1/2 = 40 s) than in isolated outer segments (t1/2 = 7 s), which suggests there is more effective buffering in OS-IS. (b) The GTP decrease becomes detectable only at intensities greater than those required to saturate the photoresponse. As the intensity of a continuous light is increased over 4 log units, GTP levels decrease linearly with log intensity by as much as 60%. GTP is reduced to steady intermediate levels during extended illumination of intermediate intensity. (c) At levels of illumination bleaching greater than 0.003% of the rhodopsin, a decrease in ATP levels becomes detectable. (d) Following a flash, GTP levels fall and then rise with a recovery time dependent on the intensity of the flash. (e) After both 0.2 and 2% flash bleaches, the recovery of GTP levels parallels the recovery of light sensitivity, which is slower than the recovery of the dark current. This raises the possibility of a link between GTP levels and light sensitivity.

MeSH Terms
Adenosine Triphosphate/metabolism Animals Anura Dark Adaptation Electrophysiology Guanosine Triphosphate/metabolism Light Photoreceptor Cells/metabolism,physiology,radiation effects
Chemicals
Guanosine Triphosphate Adenosine Triphosphate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Biernbaum M S
Bownds M D
References (44)
44 references, click to expand
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Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.85.1.107
Published
1985-01-00
Pages
107-21
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2215811
Subset
IM
External Links
PubMed source DOI: 10.1085/jgp.85.1.107 PMC Full Text View journal details
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