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PMID: 3871471 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Frog rod outer segments with attached inner segment ellipsoids as an in vitro model for photoreceptors on the retina.

The Journal of general physiology ·Vol. 85 ·No. 1 ·1985-01-00 ·Pages 83-105

Biernbaum MS, Bownds MD

Abstract

Purified suspensions of frog rod outer segments still attached to the mitochondria-rich inner segment portion of the receptor cell (OS-IS) can be obtained in quantities (0.1 mg/retina) sufficient for chemical analysis. In oxygenated glucose-bicarbonate Ringer's medium with added Percoll, they display normal dark currents, light sensitivity, and photocurrent kinetics for several hours. Two millimolar cytoplasmic levels of ATP and GTP are maintained, fivefold higher than in isolated OS. The levels are not altered by abolition of the dark current with ouabain. Nucleoside triphosphates are more effectively buffered than in isolated OS, and their levels remain constant during changes in external calcium levels. 32Pi is incorporated into endogenous ATP and GTP pools twice as efficiently as in isolated OS, and is used in the phosphorylation of rhodopsin. OS-IS take up and release 45Ca++ by Na+-, Ca++-, and IBMX-sensitive mechanisms. Illumination causes release of 45Ca++, which confirms retinal studies by other groups using Ca++-sensitive electrodes. Thus, OS-IS suspensions model the behavior of photoreceptors still attached to the living retina. Their availability permits the simultaneous assay and correlation of electrophysiological and chemical changes occurring during excitation and adaptation.

MeSH Terms
Adenosine Triphosphate/metabolism Animals Calcium/pharmacology Guanosine Triphosphate/metabolism In Vitro Techniques Light Models, Biological Photoreceptor Cells/metabolism,physiology,ultrastructure Rana catesbeiana Ranidae Retina/metabolism,physiology Rod Cell Outer Segment/metabolism,physiology,radiation effects
Chemicals
Guanosine Triphosphate Adenosine Triphosphate Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Biernbaum M S
Bownds M D
References (43)
43 references, click to expand
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Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.85.1.83
Published
1985-01-00
Pages
83-105
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2215816
Subset
IM
Grants
NEI NIH HHS · EY-00463 · United States
External Links
PubMed source DOI: 10.1085/jgp.85.1.83 PMC Full Text View journal details
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