Rapid formation of inositol 1,3,4,5-tetrakisphosphate and inositol 1,3,4-trisphosphate in rat parotid glands may both result indirectly from receptor-stimulated release of inositol 1,4,5-trisphosphate from phosphatidylinositol 4,5-bisphosphate.

The Biochemical journal ·Vol. 238 ·No. 2 ·1986-09-01 ·Pages 507-16

Hawkins PT, Stephens L, Downes CP

Abstract

Addition of 1 mM-carbachol to [3H]inositol-labelled rat parotid slices stimulated rapid formation of [3H]inositol 1,3,4,5-tetrakisphosphate, the accumulation of which reached a peak 20 s after stimulation, and then declined rapidly towards a new steady state. The initial rate of formation of inositol 1,3,4,5-tetrakisphosphate was slower than that for inositol 1,4,5-trisphosphate. The radioactivity in [3H]inositol 1,3,4,5-tetrakisphosphate fell quickly in carbachol-stimulated and then atropine-blocked parotid slices, suggesting that it is rapidly metabolized during stimulation. Parotid homogenates rapidly dephosphorylated inositol 1,4,5-trisphosphate, inositol 1,3,4,5-tetrakisphosphate and, less rapidly, inositol 1,3,4-trisphosphate. Inositol 1,3,4,5-tetrakisphosphate was specifically hydrolysed to a compound with the chromatographic properties of inositol 1,3,4-trisphosphate. The only 3H-labelled phospholipids that we could detect in parotid slices labelled with [3H]inositol for 90 min were phosphatidylinositol, phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate. Parotid homogenates synthesized inositol tetrakisphosphate from inositol 1,4,5-trisphosphate. This activity was dependent on the presence of ATP. We suggest that, during carbachol stimulation of parotid slices, the key event in inositol lipid metabolism is the activation of phosphatidylinositol 4,5-bisphosphate-specific phospholipase C. The inositol 1,4,5-trisphosphate thus liberated is metabolized in two distinct ways; by direct hydrolysis of the 5-phosphate to form inositol 1,4-bisphosphate and by phosphorylation to form inositol 1,3,4,5-tetrakisphosphate and hence, by hydrolysis of this tetrakisphosphate, to form inositol 1,3,4-trisphosphate.

MeSH Terms

Animals Calcium Channels Carbachol/pharmacology Chromatography, High Pressure Liquid Hydrolysis In Vitro Techniques Inositol 1,4,5-Trisphosphate Inositol 1,4,5-Trisphosphate Receptors Inositol Phosphates/metabolism Parotid Gland/drug effects,metabolism Phosphatidylinositol 4,5-Diphosphate Phosphatidylinositols/metabolism Phospholipids/metabolism Phosphotransferases/metabolism Phosphotransferases (Alcohol Group Acceptor) Rats Receptors, Cell Surface/metabolism Receptors, Cytoplasmic and Nuclear Sugar Phosphates/metabolism

Chemicals

Calcium Channels Inositol 1,4,5-Trisphosphate Receptors Inositol Phosphates Phosphatidylinositol 4,5-Diphosphate Phosphatidylinositols Phospholipids Receptors, Cell Surface Receptors, Cytoplasmic and Nuclear Sugar Phosphates inositol-1,3,4,5-tetrakisphosphate Inositol 1,4,5-Trisphosphate Carbachol Phosphotransferases Phosphotransferases (Alcohol Group Acceptor) Inositol 1,4,5-trisphosphate 3-kinase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Hawkins P T

Stephens L

Downes C P

References (24)

24 references, click to expand

Rapid formation of inositol 1,3,4,5-tetrakisphosphate following muscarinic receptor stimulation of rat cerebral cortical slices.
Biochem J. 1985 Nov 15;232(1):211-5 PMID: 4084229
Inositol trisphosphates in carbachol-stimulated rat parotid glands.
Biochem J. 1984 Oct 1;223(1):237-43 PMID: 6333870
Alkaline O leads to N-transacylation. A new method for the quantitative deacylation of phospholipids.
Biochem J. 1981 Apr 1;195(1):301-6 PMID: 7306057
The enzymic phosphorylation of myo-inositol.
Biochem Biophys Res Commun. 1965 May 18;19(5):598-603 PMID: 5319593
Muscarinic receptors and hydrolysis of inositol phospholipids in rat cerebral cortex and parotid gland.
J Neurochem. 1985 Feb;44(2):465-72 PMID: 2981282
Inositol 1,3,4,5-tetrakisphosphate and not phosphatidylinositol 3,4-bisphosphate is the probable precursor of inositol 1,3,4-trisphosphate in agonist-stimulated parotid gland.
Biochem J. 1986 Sep 1;238(2):501-6 PMID: 2432882
Improved method of labelling red cells with radioactive phosphorus.
Lancet. 1958 Apr 12;1(7024):766-9 PMID: 13526265
Inositol trisphosphate, a novel second messenger in cellular signal transduction.
Nature. 1984 Nov 22-28;312(5992):315-21 PMID: 6095092
The polyphosphoinositide phosphodiesterase of erythrocyte membranes.
Biochem J. 1981 Jul 15;198(1):133-40 PMID: 6275838
The enzymic formation of myoinositol 1:2-cyclic phosphate from phosphatidylinositol.
Biochem J. 1971 May;122(4):605-7 PMID: 4330852
The inositol trisphosphate phosphomonoesterase of the human erythrocyte membrane.
Biochem J. 1982 Apr 1;203(1):169-77 PMID: 6285891
Stepwise enzymatic dephosphorylation of inositol 1,4,5-trisphosphate to inositol in liver.
Nature. 1984 Nov 22-28;312(5992):374-6 PMID: 6095097
Breakdown of polyphosphoinositides and not phosphatidylinositol accounts for muscarinic agonist-stimulated inositol phospholipid metabolism in rat parotid glands.
Biochem J. 1983 Dec 15;216(3):633-40 PMID: 6320795
Phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate: lipids in search of a function.
Cell Calcium. 1982 Oct;3(4-5):467-502 PMID: 6297743
Metabolism of inositol phosphates in parotid cells: implications for the pathway of the phosphoinositide effect and for the possible messenger role of inositol trisphosphate.
Life Sci. 1984 Apr 2;34(14):1347-55 PMID: 6323902
A rapid method of total lipid extraction and purification.
Can J Biochem Physiol. 1959 Aug;37(8):911-7 PMID: 13671378
The labelling of polyphosphoinositides with [32P]Pi and the accumulation of inositol phosphates in vasopressin-stimulated hepatocytes.
Biochem J. 1986 Sep 1;238(2):491-9 PMID: 3026353
The role of protein kinase C in cell surface signal transduction and tumour promotion.
Nature. 1984 Apr 19-25;308(5961):693-8 PMID: 6232463
Inositol 1,4,5-trisphosphate and inositol 1,3,4-trisphosphate formation in Ca2+-mobilizing-hormone-activated cells.
Biochem J. 1985 Nov 15;232(1):237-43 PMID: 3002326
Inositol cyclic phosphates are produced by cleavage of phosphatidylphosphoinositols (polyphosphoinositides) with purified sheep seminal vesicle phospholipase C enzymes.
Proc Natl Acad Sci U S A. 1985 Jun;82(12):4013-7 PMID: 2987959
Metabolism of inositol 1,4,5-trisphosphate and inositol 1,3,4-trisphosphate in rat parotid glands.
Biochem J. 1985 Jul 15;229(2):505-11 PMID: 2994638
Receptor-mediated net breakdown of phosphatidylinositol 4,5-bisphosphate in parotid acinar cells.
Biochem J. 1982 Sep 15;206(3):555-60 PMID: 6184051
Isolation of a phosphomonoesterase from human platelets that specifically hydrolyzes the 5-phosphate of inositol 1,4,5-trisphosphate.
J Biol Chem. 1985 Jul 5;260(13):7868-74 PMID: 2989264
The inositol tris/tetrakisphosphate pathway--demonstration of Ins(1,4,5)P3 3-kinase activity in animal tissues.
Nature. 1986 Apr 17-23;320(6063):631-4 PMID: 3010126

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2380507

Published

1986-09-01

Pages

507-16

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1147163

Subset

External Links

PubMed source DOI: 10.1042/bj2380507 PMC Full Text View journal details

Analysis Services

Rapid formation of inositol 1,3,4,5-tetrakisphosphate and inositol 1,3,4-trisphosphate in rat parotid glands may both result indirectly from receptor-stimulated release of inositol 1,4,5-trisphosphate from phosphatidylinositol 4,5-bisphosphate.

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