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PMID: 2832699 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Organisation of the ribosomal RNA genes in Streptomyces coelicolor A3(2).

Molecular & general genetics : MGG ·Vol. 211 ·No. 2 ·1988-02-00 ·Pages 191-6

Baylis HA, Bibb MJ

Abstract

Using Southern hybridisation of radiolabelled purified ribosomal RNAs to genomic DNA the ribosomal RNA genes of Streptomyces coelicolor A3(2) were shown to be present in six gene sets. Each gene set contains at least one copy of the 5 S, 16 S and 23 S sequences and in at least two cases these are arranged in the order 16 S - 23 S - 5 S. Three gene sets, rrnB, rrnD and rrnF, were isolated by screening a lambda library of S. coelicolor A3(2) DNA. The restriction map of one of these, rrnD, was determined and the nucleotide sequences corresponding to the three rRNAs were localised by Southern hybridisation. The gene order in rrnD is 16 S - 23 S - 5 S.

MeSH Terms
DNA Restriction Enzymes DNA, Fungal/genetics,isolation & purification Genes, Bacterial Molecular Weight Nucleic Acid Hybridization RNA, Ribosomal/genetics Streptomyces/genetics
Chemicals
DNA, Fungal RNA, Ribosomal DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Baylis H A
John Innes Institute, Norwich, UK.
Bibb M J
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330593
Published
1988-02-00
Pages
191-6
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330593
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