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PMID: 338587 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Cloning of Beneckea genes in Escherichia coli.

Journal of bacteriology ·Vol. 133 ·No. 1 ·1978-01-00 ·Pages 354-63

Lamfrom H, Sarabhai A, Abelson J

Abstract

Genes from Beneckea harveyi, a luminescent marine bacterium, were cloned in Escherichia coli. This was done by producing randomly sheared fragments of Beneckea DNA and inserting them into the EcoRI site of plasmid pMB9 by the adenine-thymine joining procedure. The hybrid plasmids were used to transform E. coli C600 SF8. Among the transformants selected for tetracycline resistance, one clone that appeared to complement a leucine tb mutation was identified. The transformants were screened for the presence of Beneckea 5S genes. Four of these clones were analyzed in detail by hybridization with 16S, 23S, and 4S Beneckea RNA. The observations suggest that the ribosomal genes in Beneckea are linked, but are present in a different order than those in E. coli.

MeSH Terms
DNA, Bacterial/genetics DNA, Recombinant Escherichia coli/genetics Genes Microscopy, Electron Plasmids RNA, Ribosomal/genetics Transcription, Genetic Transformation, Genetic Vibrio/genetics Vibrionaceae/genetics
Chemicals
DNA, Bacterial DNA, Recombinant RNA, Ribosomal
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lamfrom H
Sarabhai A
Abelson J
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22 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.133.1.354-363.1978
Published
1978-01-00
Pages
354-63
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC222014
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.133.1.354-363.1978 PMC Full Text View journal details
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