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PMID: 2831372 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of the Epstein-Barr virus gp85 gene.

Journal of virology ·Vol. 62 ·No. 4 ·1988-04-00 ·Pages 1101-7

Heineman T, Gong M, Sample J, Kieff E

Abstract

The Epstein-Barr virus glycoprotein gp85 has been mapped to the Epstein-Barr virus DNA open reading frame BXLF2 (R. Baer, A. Bankier, M. Biggin, P. Deininger, P. Farrell, T. Gibson, G. Hatfull, G. Hudson, S. Stachwell, C. Sequin, P. Tufnell, and B. Barrell, Nature [London] 310:207-211, 1984). A gp85-specific monoclonal antibody reacts with the BXLF2 in vitro transcription-translation product. The monoclonal antibody also precipitates an 85-kilodalton protein from rodent cells transfected with the BXLF2 open reading frame DNA. In these cells, gp85 localizes to the cytoplasm and nuclear rim rather than to the plasma membrane as in lymphocytes. Northern (RNA) blot hybridization and analysis of a cDNA clone containing BXLF2 indicate that gp85 is translated from an unspliced, late, 2.5-kilobase transcript. Similarities between the predicted amino acid sequences of gp85 and herpes simplex virus gH (D. McGeoch and A. Davison, Nucleic Acids Res. 14:4281-4292, 1986) are noted.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Monoclonal Cell Line Cloning, Molecular DNA Restriction Enzymes DNA, Viral/genetics Fluorescent Antibody Technique Genes, Viral Glycoproteins/analysis,genetics Herpesvirus 4, Human/genetics Humans Immunoassay Nucleic Acid Hybridization Protein Biosynthesis RNA, Viral/analysis Repetitive Sequences, Nucleic Acid Software Transcription, Genetic Viral Envelope Proteins/analysis,genetics
Chemicals
Antibodies, Monoclonal DNA, Viral Glycoproteins RNA, Viral Viral Envelope Proteins DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Heineman T
Kovler Viral Oncology Laboratories, University of Chicago, Illinois 60637.
Gong M
Sample J
Kieff E
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.62.4.1101-1107.1988
Published
1988-04-00
Pages
1101-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253115
Subset
IM
Grants
NCI NIH HHS · CA 07967 · United States
NIGMS NIH HHS · GM 07218 · United States
NICHD NIH HHS · HD 07009-13 · United States
External Links
PubMed source DOI: 10.1128/JVI.62.4.1101-1107.1988 PMC Full Text View journal details
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