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PMID: 2819822 Published · ppublish English Journal Article

S1 nuclease sensitivity of a double-stranded telomeric DNA sequence.

Nucleic acids research ·Vol. 15 ·No. 15 ·1987-08-11 ·Pages 6273-92

Budarf M, Blackburn E

Abstract

We examined structural properties of poly d(C4A2).d(T2G4), the telomeric DNA sequence of the ciliated protozoan Tetrahymena. Under conditions of high negative supercoiling, poly d(C4A2).d(T2G4) inserted in a circular plasmid vector was preferentially sensitive to digestion with S1 nuclease. Only the C4A2 strand was sensitive to first-strand S1 cutting, with a markedly skewed pattern of hypersensitive sites in tracts of either 46 or 7 tandem repeats. Linear poly d(C4A2).(T2G4) showed no preferential S1 sensitivity, no circular dichroism spectra indicative of a Z-DNA conformation, no unusual Tm, and no unusual migration in polyacrylamide gel electrophoresis. The S1 nuclease sensitivity properties are consistent with a model proposed previously for supercoiled poly d(CT).d(AG) (Pulleyblank et al., Cell 42:271-280, 1985), consisting of a double-stranded, protonated, right-handed underwound helix. We propose that this structure is shared by related telomeric sequences and may play a role in their biological recognition.

MeSH Terms
Base Sequence Circular Dichroism DNA/analysis Electrophoresis, Polyacrylamide Gel Endonucleases/pharmacology Nucleic Acid Conformation Plasmids Repetitive Sequences, Nucleic Acid Single-Strand Specific DNA and RNA Endonucleases
Chemicals
DNA Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Budarf M
Blackburn E
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43 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.15.6273
Published
1987-08-11
Pages
6273-92
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306083
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.15.6273 PMC Full Text View journal details
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