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PMID: 2780561 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Conservation of the human telomere sequence (TTAGGG)n among vertebrates.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 18 ·1989-09-00 ·Pages 7049-53

Meyne J, Ratliff RL, Moyzis RK

Abstract

To determine the evolutionary origin of the human telomere sequence (TTAGGG)n, biotinylated oligodeoxynucleotides of this sequence were hybridized to metaphase spreads from 91 different species, including representative orders of bony fish, reptiles, amphibians, birds, and mammals. Under stringent hybridization conditions, fluorescent signals were detected at the telomeres of all chromosomes, in all 91 species. The conservation of the (TTAGGG)n sequence and its telomeric location, in species thought to share a common ancestor over 400 million years ago, strongly suggest that this sequence is the functional vertebrate telomere.

MeSH Terms
Animals Base Sequence Biological Evolution Chromosomes Chromosomes, Human Humans Molecular Sequence Data Nucleic Acid Hybridization Repetitive Sequences, Nucleic Acid Vertebrates/genetics
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Meyne J
Genetics Group, LS-3, Los Alamos National Laboratory, NM 87545.
Ratliff R L
Moyzis R K
References (25)
25 references, click to expand
  1. The repeat sequence of a hermit crab satellite deoxyribonucleic acid is (-T-A-G-G-)n-(-A-T-C-C-)n.
    Biochemistry. 1974 Sep 10;13(19):3930-7 PMID: 4607234
  2. Cloning human telomeric DNA fragments into Saccharomyces cerevisiae using a yeast-artificial-chromosome vector.
    Proc Natl Acad Sci U S A. 1989 Aug;86(16):6240-4 PMID: 2668959
  3. A tandemly repeated sequence at the termini of the extrachromosomal ribosomal RNA genes in Tetrahymena.
    J Mol Biol. 1978 Mar 25;120(1):33-53 PMID: 642006
  4. Circular dichroism spectra show that repeating dinucleotide DNAs may form helices in which every other base is looped out.
    Nucleic Acids Res. 1980 Aug 25;8(16):3695-707 PMID: 7433104
  5. A family of inverted repeat sequences and specific single-strand gaps at the termini of the Physarum rDNA palindrome.
    Cell. 1980 Dec;22(3):875-86 PMID: 7460016
  6. All gene-sized DNA molecules in four species of hypotrichs have the same terminal sequence and an unusual 3' terminus.
    Proc Natl Acad Sci U S A. 1981 May;78(5):3015-9 PMID: 6265931
  7. Repeated hexanucleotide C-C-C-C-A-A is present near free ends of macronuclear DNA of Tetrahymena.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12):7436-9 PMID: 6950385
  8. DNA sequences of telomeres maintained in yeast.
    Nature. 1984 Jul 12-18;310(5973):154-7 PMID: 6330571
  9. The molecular structure of centromeres and telomeres.
    Annu Rev Biochem. 1984;53:163-94 PMID: 6383193
  10. Identification of a specific telomere terminal transferase activity in Tetrahymena extracts.
    Cell. 1985 Dec;43(2 Pt 1):405-13 PMID: 3907856
  11. Rates of DNA sequence evolution differ between taxonomic groups.
    Science. 1986 Mar 21;231(4744):1393-8 PMID: 3082006
  12. The telomeres of the linear mitochondrial DNA of Tetrahymena thermophila consist of 53 bp tandem repeats.
    Cell. 1986 Sep 12;46(6):873-83 PMID: 3019555
  13. Telomeric site position heterogeneity in macronuclear DNA of Paramecium primaurelia.
    Nucleic Acids Res. 1987 Feb 25;15(4):1717-28 PMID: 3029725
  14. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors.
    Science. 1987 May 15;236(4803):806-12 PMID: 3033825
  15. Human chromosome-specific repetitive DNA sequences: novel markers for genetic analysis.
    Chromosoma. 1987;95(6):375-86 PMID: 3677921
  16. The telomere terminal transferase of Tetrahymena is a ribonucleoprotein enzyme with two kinds of primer specificity.
    Cell. 1987 Dec 24;51(6):887-98 PMID: 3319189
  17. Telomeric DNA oligonucleotides form novel intramolecular structures containing guanine-guanine base pairs.
    Cell. 1987 Dec 24;51(6):899-908 PMID: 3690664
  18. Eukaryotic nuclear telomeres: molecular fossils of the RNP world?
    Cell. 1988 Jan 29;52(2):155-8 PMID: 2449282
  19. Isolation of a higher eukaryotic telomere from Arabidopsis thaliana.
    Cell. 1988 Apr 8;53(1):127-36 PMID: 3349525
  20. Telomeric repeat from T. thermophila cross hybridizes with human telomeres.
    Nature. 1988 Apr 14;332(6165):656-9 PMID: 2833706
  21. Telomere terminal transferase activity in the hypotrichous ciliate Oxytricha nova and a model for replication of the ends of linear DNA molecules.
    Nucleic Acids Res. 1988 Jul 25;16(14B):6953-72 PMID: 3136437
  22. A highly conserved repetitive DNA sequence, (TTAGGG)n, present at the telomeres of human chromosomes.
    Proc Natl Acad Sci U S A. 1988 Sep;85(18):6622-6 PMID: 3413114
  23. A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis.
    Nature. 1989 Jan 26;337(6205):331-7 PMID: 2463488
  24. Recombination occurs during telomere formation in yeast.
    Nature. 1989 Feb 2;337(6206):429-33 PMID: 2536898
  25. Circular dichroism evidence for G-U and G-T base pairing in poly[r(G-U)] and poly[d(G-T)].
    Biopolymers. 1977 Jun;16(6):1331-42 PMID: 880358
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.18.7049
Published
1989-09-00
Pages
7049-53
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297991
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.18.7049 PMC Full Text View journal details
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