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PMID: 3677921 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Human chromosome-specific repetitive DNA sequences: novel markers for genetic analysis.

Chromosoma ·Vol. 95 ·No. 6 ·1987-00-00 ·Pages 375-86

Moyzis RK, Albright KL, Bartholdi MF, Cram LS, Deaven LL, Hildebrand CE, Joste NE, Longmire JL, Meyne J, Schwarzacher-Robinson T

Abstract

Two recombinant DNA clones that are localized to single human chromosomes were isolated from a human repetitive DNA library. Clone pHuR 98, a variant satellite 3 sequence, specifically hybridizes to chromosome position 9qh. Clone pHuR 195, a variant satellite 2 sequence, specifically hybridizes to chromosome position 16qh. These locations were determined by fluorescent in situ hybridization to metaphase chromosomes, and confirmed by DNA hybridizations to human chromosomes sorted by flow cytometry. Pulsed field gel electrophoresis analysis indicated that both sequences exist in the genome as large DNA blocks. In situ hybridization to intact interphase nuclei showed a well-defined, localized organization for both DNA sequences. The ability to tag specific human autosomal chromosomes, both at metaphase and in interphase nuclei, allows novel molecular cytogenetic analyses in numerous basic research and clinical studies.

MeSH Terms
Base Sequence Cells, Cultured Chromosome Mapping Chromosomes, Human Cloning, Molecular Humans Karyotyping Male Nucleic Acid Hybridization Repetitive Sequences, Nucleic Acid Skin/cytology
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Moyzis R K
Genetics Group, Los Alamos National Laboratory, University of California, NM 87545.
Albright K L
Bartholdi M F
Cram L S
Deaven L L
Hildebrand C E
Joste N E
Longmire J L
Meyne J
Schwarzacher-Robinson T
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00333988
Published
1987-00-00
Pages
375-86
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
Grants
NCRR NIH HHS · RR01315 · United States
Databases
GENBANK
M20262, M20263
External Links
PubMed source DOI: 10.1007/BF00333988 View journal details
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