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PMID: 27254317 Published · epublish English Journal Article

Gut Microbiota Conversion of Dietary Ellagic Acid into Bioactive Phytoceutical Urolithin A Inhibits Heme Peroxidases.

PloS one ·Vol. 11 ·No. 6 ·2016-00-00 ·Pages e0156811

Saha P, Yeoh BS, Singh R, Chandrasekar B, Vemula PK, Haribabu B, Vijay-Kumar M, Jala VR

Abstract

Numerous studies signify that diets rich in phytochemicals offer many beneficial functions specifically during pathologic conditions, yet their effects are often not uniform due to inter-individual variation. The host indigenous gut microbiota and their modifications of dietary phytochemicals have emerged as factors that greatly influence the efficacy of phytoceutical-based intervention. Here, we investigated the biological activities of one such active microbial metabolite, Urolithin A (UA or 3,8-dihydroxybenzo[c]chromen-6-one), which is derived from the ellagic acid (EA). Our study demonstrates that UA potently inhibits heme peroxidases i.e. myeloperoxidase (MPO) and lactoperoxidase (LPO) when compared to the parent compound EA. In addition, chrome azurol S (CAS) assay suggests that EA, but not UA, is capable of binding to Fe3+, due to its catechol-like structure, although its modest heme peroxidase inhibitory activity is abrogated upon Fe3+-binding. Interestingly, UA-mediated MPO and LPO inhibition can be prevented by innate immune protein human NGAL or its murine ortholog lipocalin 2 (Lcn2), implying the complex nature of host innate immunity-microbiota interactions. Spectral analysis indicates that UA inhibits heme peroxidase-catalyzed reaction by reverting the peroxidase back to its inactive native state. In support of these in vitro results, UA significantly reduced phorbol myristate acetate (PMA)-induced superoxide generation in neutrophils, however, EA failed to block the superoxide generation. Treatment with UA significantly reduced PMA-induced mouse ear edema and MPO activity compared to EA treated mice. Collectively, our results demonstrate that microbiota-mediated conversion of EA to UA is advantageous to both host and microbiota i.e. UA-mediated inhibition of pro-oxidant enzymes reduce tissue inflammation, mitigate non-specific killing of gut bacteria, and abrogate iron-binding property of EA, thus providing a competitive edge to the microbiota in acquiring limiting nutrient iron and thrive in the gut.

MeSH Terms
Animals Biocatalysis/drug effects Bone Marrow Cells/cytology Coumarins/chemical synthesis,metabolism,pharmacology Diet Edema/pathology Ellagic Acid/metabolism Gastrointestinal Microbiome/drug effects Heme/metabolism Humans Immunity, Innate/drug effects Iron/pharmacology Iron Chelating Agents/pharmacology Lactoperoxidase/antagonists & inhibitors,metabolism Lipocalin-2/metabolism Mice, Inbred C57BL Neutrophils/drug effects,metabolism Peroxidase/antagonists & inhibitors,metabolism Reactive Oxygen Species/metabolism Tetradecanoylphorbol Acetate/pharmacology Time Factors
Chemicals
Coumarins Iron Chelating Agents Lipocalin-2 Reactive Oxygen Species 3,8-dihydroxy-6H-dibenzo(b,d)pyran-6-one Ellagic Acid Heme Iron Lactoperoxidase Peroxidase Tetradecanoylphorbol Acetate
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Saha Piu
Department of Nutritional Sciences, The Pennsylvania State University, University Park, Pennsylvania, United States of America.
Yeoh Beng San
Department of Nutritional Sciences, The Pennsylvania State University, University Park, Pennsylvania, United States of America.
Singh Rajbir
Department of Microbiology and Immunology, James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky, United States of America.
Chandrasekar Bhargavi
Institute for Stem Cell Biology and Regenerative Medicine (inStem), UAS-GKVK Campus, Bellary Road, Bangalore, Karnataka, India.
Vemula Praveen Kumar
Institute for Stem Cell Biology and Regenerative Medicine (inStem), UAS-GKVK Campus, Bellary Road, Bangalore, Karnataka, India. | Ramalingaswami ReEntry Fellow, Dept. of Biotechnology, Govt. of India.
Haribabu Bodduluri
Department of Microbiology and Immunology, James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky, United States of America.
Vijay-Kumar Matam
Department of Nutritional Sciences, The Pennsylvania State University, University Park, Pennsylvania, United States of America. | Department of Medicine, The Pennsylvania State University Medical Center, Hershey, Pennsylvania, United States of America.
Jala Venkatakrishna R
Department of Microbiology and Immunology, James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky, United States of America.
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
Published
2016-00-00
Epub
2016-00-02
Pages
e0156811
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC4890745
Subset
IM
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