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PMID: 2687211 Published · ppublish English Journal Article Review

Methods for the study of liver cell heterogeneity.

The Histochemical journal ·Vol. 21 ·No. 9-10 ·1989-00-00 ·Pages 517-29

Katz NR

Abstract

A large number of histological, histochemical and biochemical techniques are available for studying liver cell heterogeneity. Structural differences are recognized by morphometric analyses of electron micrographs. The zonal heterogeneity of enzyme activities can be demonstrated by histochemistry and more precisely by ultramicrobiochemical assays in microdissected periportal and perivenous tissue. Immunohistochemistry is useful for quantifying and localizing proteins, especially isoenzymes, without depending on their biological activity. The zonal quantification of specific mRNA can be achieved by in situ hybridization. The different structural and enzymic equipment of periportal and perivenous tissue found by these techniques has led to the concept of metabolic zonation. This hypothesis can be confirmed by determination of metabolic rates in perfused liver after selective zonal damage, in separated periportal and perivenous hepatocytes as well as in periportal and perivenous tissue of perfused liver by non-invasive techniques.

MeSH Terms
Animals Histocytochemistry/methods Humans Immunohistochemistry/methods Liver/cytology,metabolism,ultrastructure Microscopy, Electron/methods
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Katz N R
Institut für Klinische Chemie und Pathobiochemie, Universitätsklinikum Giessen, Germany.
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Article Info
Journal
The Histochemical journal
Abbr.
Histochem J
ISSN
0018-2214
DOI
10.1007/BF01753352
Published
1989-00-00
Pages
517-29
Language
English
Region
Netherlands
NLM ID
0163161
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01753352
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