Principle and method of kinetic microphotometric enzyme activity determination in situ.

Histochemistry ·Vol. 64 ·No. 1 ·1979-11-00 ·Pages 1-10

Pette D, Wasmund H, Wimmer M

Abstract

An advanced apparative set-up is described for multipositional microphotometric recording of histochemical enzyme reactions in cryostat sections. It consists of a computer controlled microscope photometer with scanning stage. Measurements on the same tissue section may be performed at 12 preselected positions. These are repeatedly brought into the measuring beam in several measuring cycles. The complete measuring process, storage of measuring position coordinates, movements of the stage and statistical evaluation of the data is under computer control. By use of the gel film technique, extinction changes in tetrazolium coupled enzyme reactions can be measured continuously at initial rate conditions. Measurements are performed at identical conditions and can thus be analysed as relative enzyme activities.

MeSH Terms

Animals Computers Enzymes/analysis Histocytochemistry Photomicrography/instrumentation,methods Tissue Distribution

Chemicals

Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Pette D

Wasmund H

Wimmer M

References (22)

22 references, click to expand

Microphotometric determination of enzyme activity in single cells in cryostat sections. I. Application of the gel film technique to microphotometry and studies on the intralobular distribution of succinate dehydrogenase and lactate dehydrogenase activities in rat liver.
J Histochem Cytochem. 1972 Aug;20(8):567-76 PMID: 4402953
Microphotometric studies on intraacinar enzyme distribution in rat liver.
Histochemistry. 1979 Nov;64(1):23-33 PMID: 521313
[Histophotometric quantification of enzyme reactions, examplified by acetylcholinesterase].
Acta Histochem Suppl. 1975;15:433-40 PMID: 829955
Cytospectrophotometric studies on the lactate dehydrogenase isoenzymes in functionally different neuron-neuroglia units. I. Optimal procedure conditions and topochemical comparisons in normal mice.
Acta Histochem. 1975;54(2):218-29 PMID: 814769
Microphotometric determination of enzyme activity in single cells in cryostat section. II. Succinate dehydrogenase, lactate dehydrogenase and triosephosphate dehydrogenase activities in red, intermediate and white fibers of soleus and rectus femoris muscles of rat.
J Histochem Cytochem. 1972 Aug;20(8):577-82 PMID: 5044279
Microspectrophotometric quantitation of the diaminobenzidine reaction for histochemical demonstration of cytochrome oxidase activity.
J Histochem Cytochem. 1978 Mar;26(3):157-62 PMID: 204704
Quantitative histochemical and stereological study of alkaline phosphatase in the rat thyroid gland.
Histochemistry. 1978 Nov 24;58(1-2):129-34 PMID: 730549
Cytophotometric determination of alkaline phosphatase activity of individual neutrophilic leukocytes with a biochemically calibrated model system.
J Histochem Cytochem. 1968 Nov;16(11):693-706 PMID: 5723777
Cytophotometric studies on the reaction kinetics of acid phosphatase in motoneurons marked by injection of Evans blue into the soleus muscle of the mouse.
Cell Tissue Res. 1977 Mar 9;178(2):239-47 PMID: 844078
Quantitation of tissue-bound renal aminopeptidase by a microdensitometric technique.
J Histochem Cytochem. 1966 Jan;14(1):53-63 PMID: 5935512
Letter: On the use of quantitation in histochemistry: a review of the literature.
Histochem J. 1976 Mar;8(2):205-8 PMID: 1270333
Studies on the reduction of tetrazolium salts. II. The measurement of the half reduced and fully reduced formazans of neotetrazolium chloride in tissue sections.
Histochemie. 1973 Dec 31;37(4):351-63 PMID: 4780461
Succinic dehydrogenase content of individual muscle fibers at different ages and stages of growth.
Life Sci. 1969 Aug 15;8(16):791-808 PMID: 5803374
Intracellular localization of glycolytic enzymes in cros-striated muscles of Locusta migratoria.
Biochem Biophys Res Commun. 1962 Oct 31;9:367-70 PMID: 13942961
Effects of long-term electrical stimulation on some contractile and metabolic characteristics of fast rabbit muscles.
Pflugers Arch. 1973 Feb 6;338(3):257-72 PMID: 4736724
Cytospectrophotometric study of the kinetics of histochemical reaction of GABA-transaminase in cryostat sections of rat cerebellar cortex.
Histochemistry. 1978 Aug 15;57(1):77-86 PMID: 689926
Kinetic microphotometric activity determination in enzyme containing gels and model studies with tissue sections.
Histochemistry. 1979 Nov;64(1):11-22 PMID: 521311
Direct fluorophotometric recording of enzyme kinetics in cryostat sections. Glucose 6-phosphate dehydrogenase in the endocrine mouse pancreas.
Histochemie. 1970;21(4):307-13 PMID: 4392410
The use of a recording microdensitometer for the quantitative measurement of enzyme activities inside tissue sections.
Histochemie. 1971;27(2):125-36 PMID: 4398397
Quantitative microscopy of enzyme reactions in tissue sections.
Microsc Acta. 1977 Jul;79(4):327-38 PMID: 337053
Precise cytochemical measurement of neotetrazolium formazan by scanning and integrating microdensitometry.
Histochemie. 1972;32(2):171-90 PMID: 4342864
The measurement in tissue sections of the two formazans derived from nitroblue tetrazolium in dehydrogenase reactions.
Histochem J. 1978 Nov;10(6):739-44 PMID: 730547

Article Info

Journal

Histochemistry

Abbr.

Histochemistry

ISSN

0301-5564

DOI

10.1007/BF00493350

Published

1979-11-00

Pages

1-10

Language

English

Region

Germany

NLM ID

0411300

Subset

External Links

PubMed source DOI: 10.1007/BF00493350

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Principle and method of kinetic microphotometric enzyme activity determination in situ.

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